Nodal信号通路相关基因NOMO1在斑马鱼早期胚胎发育过程中的表达

发布时间：2018-01-04 17:37

本文关键词：Nodal信号通路相关基因NOMO1在斑马鱼早期胚胎发育过程中的表达　出处：《南京医科大学学报(自然科学版)》2013年09期 　论文类型：期刊论文

【摘要】：目的:研究斑马鱼NOMO1基因在早期胚胎发育过程中的时空表达情况。方法:首先克隆斑马鱼NOMO1同源基因全长cDNA,然后运用半定量RT-PCR技术初步检测NOMO1基因在斑马鱼早期胚胎的表达情况,最后通过原位杂交技术检测NOMO1基因在斑马鱼早期胚胎发育过程中的时空表达情况。结果:通过半定量RT-PCR及原位杂交技术显示NOMO1从受精卵时期开始即有表达,在24 hpf(受精后24 h)之前,NOMO1基因表达广泛,并主要集中在中、内胚层。从24 hpf开始其表达主要集中于神经系统如前脑、中脑、小脑、脊索前板、眼和耳囊等处。在心脏处也有少量表达。结论:NOMO1基因对斑马鱼早期胚胎发育具有一定的作用,推测NOMO1基因影响早期心脏发育。
[Abstract]:Objective: to study the temporal and spatial expression of zebrafish NOMO1 gene during early embryonic development. Methods: first, the full-length cDNA of zebrafish NOMO1 homologous gene was cloned. The expression of NOMO1 gene in zebrafish embryos was detected by semi-quantitative RT-PCR. Finally, in situ hybridization technique was used to detect the temporal and spatial expression of NOMO1 gene during the early embryonic development of zebrafish. Semi-quantitative RT-PCR and in situ hybridization showed that NOMO1 was expressed from fertilized egg stage. NOMO1 gene was widely expressed in mesoderm before 24 h (24 h after fertilization), and mainly in mesoderm. From 24 hpf onwards, the expression of NOMO1 was mainly concentrated in nervous system such as forebrain. In the midbrain, cerebellum, anterior chordal plate, eye and ear sac, there is also a little expression in the heart. Conclusion: NOMO1 gene may play an important role in the early embryonic development of zebrafish. It is speculated that NOMO1 gene affects early cardiac development.
【作者单位】：南京医科大学第一附属医院心血管内科;
【基金】：国家自然科学基金青年科学基金资助(30901473)
【分类号】：R363
【正文快照】： Nodal信号通路是转化生长因子(TGF-β)家族中重要成员之一,它对脊椎动物胚胎发育具有重要的作用,在中、内胚层的分化、背腹轴的决定、神经外胚层诱导和分化以及左右不对称性的建立过程中Nodal信号均发挥了极大的调控效用[1-2]。并且有报道还提出Nodal信号促进心室心肌的分化,

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