复方麦芽丸对PCOS模型大鼠卵巢组织促性腺激素及类固醇激素合成酶表达的影响

发布时间：2018-01-04 18:42

本文关键词：复方麦芽丸对PCOS模型大鼠卵巢组织促性腺激素及类固醇激素合成酶表达的影响　出处：《重庆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:本研究通过观察复方麦芽丸(Compound Malt Pills,CMP)对多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠动情周期变化、卵巢组织形态学变化,卵巢组织促卵泡激素(Follicle-stimulating hormone,FSH)、黄体生成素(Luteinizing hormone,LH)、3β-羟甾脱氢酶(3β-hydroxysteroid dehydrogenase,3β-HSD)和细胞色素P450酶(Cytochrome-P450,CYP450)表达的影响,探讨复方麦芽丸治疗多囊卵巢综合征的作用机理,为临床应用提供实验依据。方法:选用9日龄SD雌性大鼠60只,随机分为正常组(n=10)和造模组(n=50),造模组大鼠颈背部皮下注射丙酸睾酮建立PCOS模型,造模成功的大鼠随机分为模型组,达英-35组和CMP低、中、高剂量组(n=9)。各治疗组以相应药物灌胃,正常组及模型组灌以等量的蒸馏水,各组大鼠均连续灌胃21d。治疗结束后瑞氏染色法观察大鼠动情周期变化,行HE染色观察大鼠卵巢组织形态,运用免疫组化、Western blot、RT-PCR或q PCR法检测卵巢组织中FSH、LH、3β-HSD、CYP450蛋白及基因的表达。结果:(1)造模成功的大鼠失去规律的动情周期变化,各治疗组性周期有不同程度恢复。(2)模型组大鼠卵巢组织可见较多囊性扩张的卵泡,颗粒细胞层数减少,各治疗组囊性卵泡直径不同程度减小,颗粒细胞层增加,黄体数量增多。(3)模型组LH表达显著升高(P0.01),FSH表达显著降低(P0.01),复方麦芽丸各剂量组LH表达显著降低(P0.01),FSH表达显著升高(P0.01)。(4)模型组3β-HSD表达显著升高(P0.01),CYP450表达显著降低(P0.01),复方麦芽丸各治疗组3β-HSD表达显著降低(P0.01),CYP450表达显著升高(P0.01)。结论:复方麦芽丸治疗PCOS可能是通过改善动情周期紊乱、卵巢多囊样改变及调控卵巢组织FSH、LH、3β-HSD、CYP450的表达实现的。
[Abstract]:Objective: This study through the observation of compound malt (Compound Malt Pills, CMP pill) on polycystic ovary syndrome (polycystic ovary, syndrome, PCOS) rat estrous cycle, ovarian morphological changes, ovarian follicle stimulating hormone (Follicle-stimulating hormone, FSH), luteinizing hormone (Luteinizing, hormone, LH), 3 beta hydroxysteroid dehydrogenase (3 P -hydroxysteroid dehydrogenase 3 beta -HSD) and cytochrome P450 enzymes (Cytochrome-P450, CYP450) expression, to explore the mechanism of compound malt pill in the treatment of polycystic ovary syndrome, and provide experimental basis for clinical application. Methods: the 9 day old female SD 60 rats were randomly divided into normal group (n=10) and model group (n=50), model group rats subcutaneous injection of testosterone propionate to establish the PCOS model, the successful model of the rats were randomly divided into model group, -35 group and CMP with low and high dose group (n=9) in the treatment. Group with corresponding drugs by gavage, the normal group and model group were fed with the same amount of distilled water, the rats were continuously administered after treatment ended gastric 21d. Wright staining method to observe the estrous cycle changes, morphological observation of rat ovarian tissue by HE staining, immunohistochemical detection of Western, blot, RT-PCR or ovarian tissue Q PCR in FSH, LH, -HSD 3 beta, CYP450 protein expression and gene. Results: (1) the changes of estrous cycle in rats without law, each group of cycles have different degree of recovery. (2) model rats with polycystic ovarian tissue expansion of the follicle reduce the number of granule cells, each treatment group, cystic follicular diameter reduced in different degree, granule cell layer increased, the number of corpus luteum increased. (3) the expression of LH in model group increased significantly (P0.01), the expression of FSH was significantly decreased (P0.01), compound malt pill each dose group LH was significantly decreased, the expression of FSH (P0.01) significant Increased (P0.01). (4) model group 3 beta -HSD expression was significantly increased (P0.01), the expression of CYP450 was significantly decreased (P0.01), malt pill in the treatment group compound 3 beta -HSD expression decreased significantly (P0.01), the expression of CYP450 was significantly increased (P0.01). Conclusion: Compound malt pill in the treatment of PCOS may be improved by estrogen cycle disorder, polycystic ovarian changes and regulation of ovarian tissue FSH, LH, -HSD 3 beta, CYP450 expression.

【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

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