甘露消毒丹及其残方对EV71感染模型小鼠的免疫调节作用

发布时间：2018-01-05 04:08

本文关键词：甘露消毒丹及其残方对EV71感染模型小鼠的免疫调节作用　出处：《湖南中医药大学》2015年博士论文　论文类型：学位论文

【摘要】：目的：探讨甘露消毒丹及其残方对EV71感染模型小鼠机体免疫炎症损伤的免疫调节作用,并进一步认识甘露消毒丹配伍的科学内涵。方法：①文献研究：从甘露消毒丹方源、临床运用情况、抗病毒物质基础等方面探讨甘露消毒丹治手足口病的有效性。②实验研究：7日龄ICR小鼠分为空白组、模型组、全方组、芳残方组、清残方组、利残方组等6组。在高温、高湿环境中腹腔注射造EV71病毒造模并使用相应药物干预治疗。用Elisa法检测小鼠靶组织中IL-10、IFN-γ、TNF-α等细胞因子变化；用流式细胞术检测小鼠脾脏淋巴细胞亚群；用Western blot法检测靶组织中IRF3蛋白表达。结果：①从文献研究来看,甘露消毒丹为叶天士所创,首载于《续名医类案》具有治疗EV71型手足口病的临床基础。②EV71感染小鼠后第5天观测临床表现,检测后肢肌肉组织形态及是否表达了EV71病毒,均符合造模标准,确认造模成功。③甘露消毒丹及其残方能调节小鼠脾脏T淋巴细胞亚群,提高IRF3表达,促进IL-10、IFN-γ的分泌,同时降低靶组织中TNF-α等炎症因子。结论：①甘露消毒丹是清代著名医家叶天士所创之方,此方选药精当,配伍严谨,治疗EV71感染湿热证手足口病疗效显著,值得推广。②甘露消毒丹能通过调节脾脏淋巴细胞亚群,显著提高机体抗EV71病毒的免疫功能。③甘露消毒丹能诱导IRF3的表达,刺激免疫细胞分泌IFN-γ干扰素提高机体的抗病毒能力,同时又能降低促炎因子TNF-α和提高抑炎IL-10细胞因子的分泌减轻炎症反应。④利残方在调节免疫功能方面有优势,清残方在控制炎症反应方面更擅长,他们之间相辅相成。甘露消毒丹中清热解毒药、利湿化浊药各有所长,各司其职,方剂配伍全面合理。
[Abstract]:Objective: to investigate the immunomodulatory effect of Ganlu Xiexian Dan (GXD) and its residual prescription on immune inflammation injury in mice infected with EV71, and to understand the scientific connotation of the compatibility of Ganlu Xiexian Dan (GXD). Methods: literature study: from the source of mannoxiao Dan, clinical application. Experimental study on the effectiveness of Gannuzhidan in the treatment of Hand-Foot-mouth Disease; ICR mice of 7 days of age were divided into blank group, model group, whole prescription group, Fang and remnant prescription group, and Qingruan recipe group. In the high temperature and high humidity environment, the EV71 virus model was made by intraperitoneal injection and the corresponding drug intervention was used. IL-10 and IFN- 纬 in the target tissues of mice were detected by Elisa method. Changes of cytokines such as TNF- 伪; The lymphocyte subsets of spleen were detected by flow cytometry. The expression of IRF3 protein in target tissues was detected by Western blot method. Results according to the literature study, Mannu disinfectant was created by Yetianshi. The clinical manifestation of mice infected with EV71 was observed on the 5th day after infection with EV71 type HFMD. The morphology and expression of EV71 virus in hind limb muscle were detected, which were in accordance with the model making standard. It was confirmed that the successful establishment of the model could regulate the spleen T lymphocyte subsets of mice with the successful establishment of 3. 3 mannose disinfectant and its residual prescription. Increase the expression of IRF3, promote the secretion of IL-10 and IFN- 纬, and decrease the inflammatory factors such as TNF- 伪 in target tissues. Conclusion 1 Mannu disinfectant Dan is the prescription of Ye Tianshi, a famous doctor in Qing Dynasty. The prescription is effective in treating hand-foot-mouth disease of EV71 infection with damp-heat syndrome. It is worth popularizing 2.Mannuxiaodan can significantly improve the immune function of the body against EV71 virus by regulating the lymphocyte subsets of spleen, and can induce the expression of IRF3. 3. Stimulating the immune cells to secrete IFN- 纬 interferon enhances the antiviral ability of the body. At the same time, it can reduce the inflammatory factor TNF- 伪 and increase the secretion of anti-inflammatory IL-10 cytokines. Qingruo prescription is better at controlling inflammatory reaction, and they complement each other. MannoxuoDan has its own strengths and advantages in heat clearing and detoxifying medicine, and it has its own advantages and responsibilities, and its compatibility is comprehensive and reasonable.
【学位授予单位】：湖南中医药大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R285.5;R-332

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