微量量热法体外表征拉米夫定对肠道特征菌群的影响

发布时间：2018-01-05 20:01

本文关键词：微量量热法体外表征拉米夫定对肠道特征菌群的影响　出处：《药学学报》2013年10期 　论文类型：期刊论文

【摘要】：采用微量量热法研究拉米夫定对3种肠道特征细菌青春双歧杆菌、痢疾志贺菌及大肠埃希菌生长代谢的影响。在不同给药剂量下,以表达功率时间曲线(热谱曲线)的特征参数生长速率常数(k)、热功率(p)、达峰时间(t)、发热量(Q)为指标,对拉米夫定影响肠道特征细菌生长代谢程度进行客观地量化评价。结果显示,拉米夫定对青春双歧杆菌的半数抑制率(IC50)为200μg·mL-1,对痢疾志贺菌的IC50大于3 000μg·mL-1,对大肠埃希菌的IC50大于6 000μg·mL-1。结果显示,拉米夫定对3种肠道特征菌群抑制效果呈现明显差异,其"敏感"程度依次为青春双歧杆菌痢疾志贺菌大肠埃希菌;研究提示长期口服拉夫米定可能破坏菌道菌群平衡,存在导致内毒素等增加、引发炎症、加速肝病病情恶化的潜在风险。
[Abstract]:The effects of lamivudine on the growth and metabolism of three enteric bacteria bifidobacterium, Shigella dysenteriae and Escherichia coli were studied by microcalorimetry. The characteristic parameters of the power time curve (thermal spectrum curve) are as follows: growth rate constant, thermal power, peak time and calorific value. The effects of lamivudine on the growth and metabolism of intestinal characteristic bacteria were evaluated objectively. The results showed that the half inhibitory rate of lamivudine on Bifidobacterium puberty was 200 渭 g 路mL-1. The IC50 of Shigella dysenteriae and Escherichia coli were more than 3 000 渭 g 路mL -1 and 6 000 渭 g 路mL -1 respectively. The inhibitory effect of lamivudine on the three enteric characteristic bacteria was obviously different, and the "sensitive" degree of lamivudine was Escherichia coli, Shigella spp., dysentery and dysentery. The results suggest that long-term oral administration of Lafmicidine may disrupt the balance of bacterial flora, lead to the increase of endotoxin, lead to inflammation and accelerate the deterioration of liver disease.
【作者单位】：解放军第三零二医院全军中药研究所;江西中医药大学;
【基金】：国家自然科学基金资助项目(81274078,81322052,81303222) 中国博士后科学基金项目资助(2013M532130)
【分类号】：R378
【正文快照】： 目前抗乙型肝炎病毒治疗药物主要有两类:α-干扰素类和核苷(酸)类似物,口服核苷(酸)类药物是目前被普遍接受的一种治疗手段。其中,拉米夫定(lamivudine)是第一个获批准的口服抗乙型肝炎病毒(HBV)的核苷类药物,具有抑制病毒迅速、疗效好等优点,其在抗HIV、HBV联合用药治疗中起

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