肺炎嗜衣原体热休克蛋白10促炎症作用及其机制的初步研究

发布时间：2018-01-07 12:13

本文关键词：肺炎嗜衣原体热休克蛋白10促炎症作用及其机制的初步研究　出处：《免疫学杂志》2013年02期 　论文类型：期刊论文

【摘要】：目的探讨肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)热休克蛋白(heat shock protein,HSP)10(CHSP10)诱导人单核细胞分泌炎症因子的作用及Toll样受体(Toll-like receptor,TLR)2、TLR4与此作用的相关性。方法以去内毒素活性的不同质量浓度CHSP10(0.5、1、5、10、20、30μg/ml)刺激THP-1细胞0、6、12、24、36、48、60 h,检测蛋白未处理组、加热处理组、去蛋白处理组中IL-1β及IL-6水平;用CHSP10刺激C3H系野生型(C3H/HeN)和TLR4缺陷型(C3H/HeJ)小鼠腹腔巨噬细胞,分别检测IL-1β及IL-6水平;用CHSP10刺激被抗TLR2/TLR4抗体处理的THP-1细胞,检测IL-1β、IL-6的变化。结果 CHSP10可诱导THP-1细胞产生炎症因子IL-1β、IL-6;CHSP10诱生C3H系野生型小鼠细胞分泌的炎症因子明显高于TLR4缺陷型小鼠细胞;用TLR2和/或TLR4抗体封闭后,CHSP10诱生的IL-1β、IL-6不同程度减少。结论 CHSP10可能作为炎症相关蛋白参与了Cpn对宿主细胞的致炎作用;并且TLR2及TLR4在该炎症刺激信号的传递过程中发挥一定的作用。
[Abstract]:Objective to investigate the heat shock protein (shock protein) of Chlamydophila pneumoniae. HSP10 / CHSP10) induced the secretion of inflammatory cytokines by human monocytes and Toll like receptor Toll-like receptor TLR2. Methods the THP-1 cells were stimulated with different concentrations of CHSP100.5 / ml and 2030 渭 g / ml, respectively. The levels of IL-1 尾 and IL-6 in untreated, heated and deproteinized groups were detected for 60 h. The peritoneal macrophages of C3H / HeN and TLR4 deficient C3H / HeJ mice were stimulated by CHSP10 and the levels of IL-1 尾 and IL-6 were measured. THP-1 cells treated with anti TLR2/TLR4 antibody were stimulated with CHSP10 to detect IL-1 尾. Results CHSP10 could induce the production of the inflammatory factor IL-1 尾 -IL-6 in THP-1 cells. The inflammatory factors secreted by C3H wild-type mice induced by CHSP10 were significantly higher than those of TLR4 deficient mice. IL-1 尾 was induced by CHSP10 after blocking with TLR2 and / or TLR4 antibody. Conclusion CHSP10 may be involved in the inflammatory effect of Cpn on host cells as an inflammatory associated protein. And TLR2 and TLR4 play a role in the transmission of inflammatory stimulation signal.
【作者单位】：南华大学微生物与免疫学教研室;湖南省疾病预防控制中心;
【基金】：国家自然科学基金(30901352);国家自然科学基金(81001318) 传染病预防控制国家重点实验室自主研究开放课题(2012SKLID308) 湖南省高校科技创新团队(湘教通[2010]53号) 湖南省研究生创新基地资助项目
【分类号】：R392.12
【正文快照】： 肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)为嗜衣原体属下的一类呼吸道病原体,是具有独特发育周期的胞内寄生菌。作为一种常见的感染因子,Cpn除可引起非典型性肺炎的暴发流行,还与冠状动脉粥样硬化等多种慢性疾病相关[1-3]。Cpn感染引起的炎症反应是多种疾病的发病基础。

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