SD大鼠寒积里实证模型的建立与评价

发布时间：2018-01-10 20:11

本文关键词：SD大鼠寒积里实证模型的建立与评价　出处：《中国药理学通报》2013年02期 　论文类型：期刊论文

【摘要】：目的在中医理论指导下建立SD大鼠寒积里实证动物模型并对其进行评价。方法应用先冰水后高岭土灌胃方法制作寒积里实证动物模型。观察模型动物的一般生物学特征变化,进行模型动物的排便和尾部温度测量实验,检测模型动物的肠神经递质、胃肠激素、cAMP和cGMP等客观指标。结果造模后,模型动物的一般生物学特征表现为:披毛蓬松、好聚集饲养笼一端、大便干燥,摄食量降低;与空白对照组比较,模型组动物的6 h排便粒数明显减少,尾部温度明显降低,胃动素、血管活性肠肽、P物质分泌紊乱,cAMP/cGMP降低,经大黄附子汤治疗后,上述症状和指标改善。结论应用先冰水后高岭土灌胃方法能复制出稳定的SD大鼠寒积里实证模型。
[Abstract]:Objective to establish and evaluate the experimental animal model of cold accumulation in SD rats under the guidance of traditional Chinese medicine theory. Changes in physical characteristics. The defecation and tail temperature of model animals were measured to detect the intestinal neurotransmitters, gastrointestinal hormones camp and cGMP. The general biological characteristics of the model animals are as follows: draped and fluffy, easy to gather and raise one end of the cage, dry stool, food intake reduced; Compared with the blank control group, the number of defecation grains in the model group was significantly decreased, the tail temperature was significantly decreased, and the motilin and vasoactive intestinal peptide substance P secretion disorder (cAMP / cGMP) was significantly decreased in the model group. The symptoms and indexes mentioned above were improved after treatment with rhubarb aconite decoction. Conclusion the stable cold accumulation model of SD rats can be replicated by using the method of ice water and kaolin.
【作者单位】：南京中医药大学药学院;南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心;南京海昌中药集团;武汉名老中医会诊中心;
【基金】：国家自然科学基金资助项目(No 81073022)
【分类号】：R-332
【正文快照】： 寒积里实证史载于《金匮要略·腹满寒疝宿食病脉证治第十》篇,原文云:“胁下偏痛,发热,其脉紧弦,此寒也,以温药下之,宜大黄附子汤。”临床表现为胁下偏痛,发热,手足厥冷,脉紧弦。盖阴寒积聚,横陈一处,虽见发热,亦是阳气被郁,难以输布。此则非温阳不能散其寒,非泻下不能去其积

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