miR-206-3p在小鼠皮肤中的表达及其作用

发布时间：2018-01-11 03:03

本文关键词：miR-206-3p在小鼠皮肤中的表达及其作用　出处：《临床检验杂志》2013年07期 　论文类型：期刊论文

【摘要】：目的对小鼠皮肤中miR-206-3p及其靶基因脑源性神经营养因子(Bdnf)进行表达与定位检测,以探讨其在皮肤发育过程中的可能作用。方法手术剪取13.5 dpc(交配后天数)、15.5 dpc、17.5 dpc胎鼠,1 dpp(出生后天数)、4 dpp乳鼠及16周龄雄鼠的背部全层皮肤,用实时荧光定量RT-PCR检测miR-206-3p与Bdnf mRNA的表达水平,western blot检测BDNF蛋白质表达水平,原位杂交与免疫组化分别检测miR-206-3p与BDNF的组织定位。结果皮肤中miR-206-3p在胚胎期持续升高,到17.5 dpc后逐渐降低,于成年鼠时回复至13.5 dpc时水平;BDNF表达趋势与miR-206-3p相反,而Bdnf mRNA在17.5 dpc至成年鼠并无显著改变。miR-206-3p的分布在皮肤发育过程中逐渐区域化,在出生后主要分布于上基部和毛囊外周,与同期BDNF表达区域毗邻但不重叠。结论 miR-206-3p可能通过转录后调控Bdnf参与了小鼠皮肤神经发育过程。
[Abstract]:Objective to detect the expression and localization of miR-206-3p and its target gene brain-derived neurotrophic factor (BDNF) in mouse skin. To explore its possible role in the course of skin development. Methods the fetal mice with 13.5 dpcs (15.5dpcl / 17.5 dpc) were cut out after mating. 1 DPP (4 dpp postnatal days after birth) and 16 weeks old male mice with full back skin. The expression level of miR-206-3p and Bdnf mRNA was detected by real-time fluorescence quantitative RT-PCR and BDNF protein was detected by western blot. The tissue localization of miR-206-3p and BDNF was detected by in situ hybridization and immunohistochemistry. It decreased gradually after 17.5 dpc and returned to 13.5 dpc in adult rats. The trend of BDNF expression was opposite to that of miR-206-3p. However, there was no significant change in the distribution of .miR-206-3p in Bdnf mRNA from 17.5 dpc to adult rats during the course of skin development. After birth, it was mainly distributed at the upper base and around the hair follicle. Conclusion miR-206-3p may play an important role in the development of mouse skin nerves through posttranscriptional regulation of Bdnf.
【作者单位】：江苏大学基础医学与医学技术学院;淮安市第二人民医院病理科;
【基金】：江苏省研究生创新计划(CXLX11_0606)
【分类号】：R329
【正文快照】： miR-206-3p作为肌肉特异性microRNA(miRNA)家族成员之一,几乎只局限表达于骨骼肌细胞[1]。Radzikinas等[2]研究发现,在小鼠胚肺早期发育中,miR-206-3p通过对脑源性神经营养因子(brain derived neurotrophic factor,Bdnf)的转录后调控,参与了肺叶远端气道平滑肌的神经支配过程

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