肿瘤坏死因子与可溶性受体之间相互作用的动力学研究

发布时间：2018-01-11 12:08

本文关键词：肿瘤坏死因子与可溶性受体之间相互作用的动力学研究　出处：《分析化学》2013年05期 　论文类型：期刊论文

【摘要】：采用表面等离子体共振(SPR)技术研究了肿瘤坏死因子(TNF)与其两种受体的胞外部分即可溶性受体(TNF sRI/Fc Chimera和TNF sRII/Fc Chimera)的相互作用的动力学性质。采用两种方式进行研究:在传感片上氨基偶联固定TNF,测定其与溶液中两种可溶性受体相互作用;在传感片上固定Protein A,通过其与嵌合蛋白TNF sRI/Fc Chimera和TNF sRII/Fc Chimera分子上Fc片段的特异性作用,将可溶性受体"捕获"固定在传感片上,再测定受体与溶液中TNF的相互作用。结果表明,两种固定方式测定的动力学参数相吻合,TNF与其可溶性受体的亲和常数KD大于10"9mol/L,远高于一般抗体-抗原间的亲和常数(KD=10"5～10"7mol/L)。
[Abstract]:The surface plasmon resonance (SPR) technique was used to study the extracellular fraction of tumor necrosis factor TNFand its two receptors. TNF sRI/Fc Chimera and TNF sRII/Fc Chimera). The kinetic properties of the interaction were studied in two ways: TNF was immobilized on the sensing chip by amino coupling. Its interaction with two soluble receptors in solution was determined. Protein A was fixed on the sensing chip. The specific action of FC fragment on chimeric protein TNF sRI/Fc Chimera and TNF sRII/Fc Chimera was obtained. The soluble receptor "capture" was immobilized on the sensing chip and the interaction between the receptor and the TNF in the solution was determined. The results showed that the kinetic parameters of the two fixation methods were in agreement with each other. The affinity constant of TNF to its soluble receptor was greater than 10 "9 mol / L, which was much higher than that of KD10" 5 "5" 7 mol / L ~ (-1).
【作者单位】：中国科学院长春应用化学研究所电分析化学国家重点实验室;
【基金】：国家自然科学基金项目(No.21175124) 吉林省科技发展计划项目(No.20125090)资助
【分类号】：R312
【正文快照】： 1引言细胞因子是免疫原、丝裂原或其刺激剂诱导多种细胞产生的能调节细胞功能的低分子量可溶性蛋白质。在免疫应答过程中,细胞因子对于细胞间相互作用、细胞的生长和分化有重要调节作用。肿瘤坏死因子(TNF)是一种重要的细胞因子,是一种免疫调节因子,分为α和β两种,在宿主的

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