脂肪乳剂灌胃加氮气损伤建立兔颈动脉粥样硬化动物模型

发布时间：2018-01-14 20:22

本文关键词：脂肪乳剂灌胃加氮气损伤建立兔颈动脉粥样硬化动物模型　出处：《中国实验方剂学杂志》2013年11期 　论文类型：期刊论文

【摘要】：目的:探索一种快速建立颈动脉粥样硬化动物模型的有效方法。方法:将30只雄性日本大耳白兔随机分为3组,分别给予正常饲料喂养、单纯脂肪乳剂ig和脂肪乳剂ig加氮气损伤(脂肪乳剂按胆固醇∶猪油∶蛋黄粉1∶5∶4配制;同时历时5 min,150 mL.min-1氮气流损伤血管内皮)。于实验第4周和第8周,检测动物血脂含量并观察颈动脉病理变化特征。结果:脂肪乳剂手术组大耳白兔4周时已经形成高脂血症,血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量分别达(17.55±5.36),(13.68±2.67)mmol.L-1较空白组显著升高(P0.01),内膜轻度增厚呈现动脉粥样硬化早期改变;8周时TC,LDL-C含量进一步升高分别达(21.13±3.59),(15.18±3.29)mmol.L-1,内膜明显增厚可见大量泡沫细胞及脂质沉积。而上述病理改变未见于空白组、脂肪乳剂组和脂肪乳剂手术组对侧的颈动脉。结论:脂肪乳剂加氮气损伤能有效的建立兔颈动脉粥样硬化模型。
[Abstract]:Objective: To explore an effective method for the rapid establishment of animal model of carotid atherosclerosis. Methods: 30 male Japanese white rabbits were randomly divided into 3 groups, were given normal diet, pure fat emulsion Ig and fat emulsion Ig and nitrogen gas injury (fat emulsion by cholesterol: Lard yolk powder: 1: 5: 4 at the same time; after 5 min, 150 mL.min-1). Nitrogen flow of vascular endothelial injury in experiment fourth and eighth weeks, blood lipid content and characteristics of animal observation of carotid artery pathological examination. Results: fat emulsion operation group rabbits at 4 weeks has formed hyperlipidemia, serum total cholesterol (TC), low density lipoprotein the content of cholesterol (LDL-C) respectively (17.55 + 5.36), (13.68 + 2.67) mmol.L-1 was significantly higher than that in blank group (P0.01), intimal thickening showed early changes of atherosclerosis; at 8 weeks TC, LDL-C content increased further respectively (21.13 + 3. 59), (15.18 + 3.29) mmol.L-1, significant intimal thickening shows a large number of foam cells and lipid deposition. The pathological changes seen in the blank group, fat emulsion group and operation group of fat emulsion on the side of the carotid artery. Conclusion: Intralipid plus nitrogen injury can establish rabbit carotid atherosclerosis model effectively.

【作者单位】：成都中医药大学药学院;中国中医科学院;
【基金】：综合性中药新药研究开发技术大平台(2009ZX09301-005)
【分类号】：R-332
【正文快照】： 颈动脉粥样硬化(atherosclerosis,AS)是一个复杂的病理生理过程,与卒中有着密切的关系,是短暂性脑缺血发作、脑梗死的主要原因之一。随着对颈AS的发病机制及药物干预作用的研究,需要建立一种简便易行、重复性好、易于评价、有较典型的AS病理改变、适于干预治疗的动物模型。以

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