突触后蛋白SAPAP4在学习和记忆中的作用初探

发布时间：2018-01-19 12:31

本文关键词： SAPAP4 突触后蛋白基因敲除小鼠情绪记忆空间记忆　出处：《华东师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：SAPAP(SAP90/PSD-95-associated protein,SAPAP)蛋白家族位于突触后致密区(PSD),它与PSD-95和Shank蛋白家族相互作用,形成了重要的突触后脚手架蛋白复合体,对于兴奋性突触的形成、稳定、可塑变化以及信号传导有重要作用。已知,SAPAP蛋白家族在中枢神经系统中分布广泛,且各亚型分布并不一致,其功能也有所侧重。而SAPAP4作为其成员之一,广泛分布于海马、大脑皮层、纹状体、杏仁核等诸多与大脑学习记忆相关的脑区,但SAPAP4在学习记忆过程中扮演何种角色目前还不清楚。本研究通过前期构建的SAPAP4基因敲除小鼠,利用多种行为学方法,重点研究SAPAP4功能缺失对小鼠学习记忆能力的影响。我们利用条件性恐惧实验和被动躲避实验对SAPAP4基因敲除小鼠的情绪性记忆能力进行了检测。结果发现,该小鼠可能存在情景恐惧记忆和线索恐惧记忆的缺陷,并在探索行为上存在异常。我们通过T迷宫和Morris水迷宫对SAPAP4基因敲除小鼠的空间学习记忆能力进行了检测。结果发现,SAPAP4基因敲除小鼠可能在空间学习和记忆能力上存在一定缺陷。另外,我们还发现SAPAP4基因敲除小鼠四月龄和七月龄的体重明显轻于对照组。我们的实验结果,为了解SAPAP4基因敲除小鼠在学习记忆中的行为表型提供了更多证据,也为进一步研究SAPAP4基因在学习和记忆中的作用和机制提供了基础。
[Abstract]:The SAPAP(SAP90/PSD-95-associated protein SAPAP(SAP90/PSD-95-associated protein family is located in the postsynaptic compact region (PSD). It interacts with PSD-95 and Shank protein family and forms an important postsynaptic scaffold protein complex which is stable for the formation of excitatory synapses. Plastic changes and signal transduction play an important role. It is known that SAPAP protein family is widely distributed in the central nervous system, and the distribution of different subtypes is not consistent. As one of its members, SAPAP4 is widely distributed in the hippocampus, cerebral cortex, striatum, amygdala and many other brain areas related to learning and memory. However, it is not clear what role SAPAP4 plays in the learning and memory process. In this study, SAPAP4 gene knockout mice were constructed in the early stage, and various behavioral methods were used. We studied the effect of SAPAP4 deficiency on learning and memory ability of mice. We examined the emotional memory ability of SAPAP4 knockout mice by using conditioned fear test and passive avoidance experiment. The test. It turns out. The mice may be deficient in situational fear memory and clue fear memory. We tested the spatial learning and memory ability of SAPAP4 knockout mice by T maze and Morris water maze. SAPAP4 knockout mice may have some defects in spatial learning and memory. We also found that SAPAP4 knockout mice were significantly lighter in weight at the age of four and seven months than in the control group. It provides more evidence for understanding the behavioral phenotype of SAPAP4 knockout mice in learning and memory, and provides a basis for further study on the role and mechanism of SAPAP4 gene in learning and memory.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R338

【相似文献】