白细胞介素-1β在SD大鼠慢性非细菌性前列腺炎模型中的表达及其意义

发布时间：2018-01-24 11:42

本文关键词： 白细胞介素-β 慢性非细菌性前列腺炎大鼠动物模型　出处：《安徽医科大学学报》2017年06期 　论文类型：期刊论文

【摘要】：目的通过构建雄性SD大鼠慢性非细菌性前列腺炎(CNP)模型研究白细胞介素-1β(IL-1β)的表达,探讨IL-1β在CNP中的可能发挥的作用。方法取3月龄雄性SD大鼠30只随机分为正常对照组和CNP模型组,每组15只。造模后通过HE染色观察两组大鼠前列腺组织炎症浸润情况,并应用Western blot法、免疫组化SABC法检测两组SD大鼠前列腺组织中IL-1β表达情况。结果 HE染色结果证实成功构建出SD大鼠慢性非细菌性前列腺炎模型,Western blot法、免疫组化法检测都显示CNP模型组IL-1β的表达水平均高于正常对照组,差异有统计学意义(P0.05)。结论IL-1β在SD大鼠CNP模型中表达水平增高,在前列腺炎症的发生发展过程中起着重要作用。
[Abstract]:Objective to study the expression of interleukin-1 尾 (IL-1 尾) in male SD rats with chronic nonbacterial prostatitis (CNP). Methods Thirty 3-month-old male SD rats were randomly divided into normal control group and CNP model group. 15 rats in each group were used to observe the inflammatory infiltration of prostate tissue by HE staining, and Western blot method was used. Immunohistochemical SABC method was used to detect the expression of IL-1 尾 in the prostatic tissues of SD rats. Results the chronic non-bacterial prostatitis model of SD rats was successfully constructed by HE staining. The expression of IL-1 尾 in CNP model group was higher than that in normal control group by Western blot method and immunohistochemical method. Conclusion the expression of IL-1 尾 in SD rat CNP model is increased and plays an important role in the occurrence and development of prostatic inflammation.
【作者单位】：安徽医科大学第一附属医院泌尿外科;安徽医科大学泌尿外科研究所;
【基金】：国家自然科学基金(编号:81401518;81370856;81470986) 国家临床重点专科(含中医专业)建设项目(2012)
【分类号】：R-332;R697.33
【正文快照】： 2017-02-05接收慢性前列腺炎(chronic prostatitis,CP)是泌尿男科的常见病和多发病,最近我国的一项大样本流行病学调查显示中国男性前列腺炎样症状发生率约为8.4%[1],其主要表现为下腹及会阴部耻骨区疼痛不适,排尿异常,并且可导致男性功能障碍和男性不育,严重影响着患者的生活

【相似文献】