旋毛虫p43与p53核酸疫苗的构建及其免疫保护性

发布时间：2018-01-29 17:03

本文关键词： 旋毛虫 p p 核酸疫苗免疫保护性　出处：《中国兽医科学》2013年03期 　论文类型：期刊论文

【摘要】：为探讨旋毛虫p43、p53基因的核酸疫苗对小鼠的免疫保护效果与免疫保护机制,将p43、p53基因分别克隆到真核表达载体pcDNA3.1(+)上,分3次肌肉注射免疫3组昆明小鼠。于三免后2周经口感染旋毛虫肌幼虫(100蚴/只),在攻虫后第7天和第35天剖杀小鼠,计算成虫减虫率、肌幼虫减虫率和繁殖力指数,评估其免疫效果。在各时间点上取小鼠血清用于检测抗体水平和脂肪酸结合蛋白(H-FABP)。结果显示,试验各组成虫减虫率与对照组相比差异显著(P0.05),LPG及RCI减虫率与对照组相比均差异极显著(P0.01);IgG抗体水平提高,与PBS组差异显著(P0.05);各组H-FABP检测结果均低于70pg/mL。结果表明旋毛虫p43与p53基因的核酸疫苗具有较好的抗旋毛虫的免疫保护效果,且两核酸疫苗对小鼠心肌无损伤。
[Abstract]:In order to investigate the protective effect and mechanism of nucleic acid vaccine p43 and p53 gene of Trichinella spiralis p43 on mice. P53 gene was cloned into eukaryotic expression vector pcDNA3.1 (), and three groups of Kunming mice were immunized by intramuscular injection. Mice were killed on the 7th and 35th day after attack, and the adult worm reduction rate, muscle larva worm reduction rate and fecundity index were calculated. To evaluate its immunological effect, serum samples were taken from mice at different time points to detect antibody level and fatty acid binding protein (H-FABPN). The results showed that: 1. Compared with the control group, the adult worm reduction rate of the test group was significantly different from that of the control group (P 0.05) and the RCI reduction rate was significantly different from the control group (P 0.01). The level of IgG antibody was higher than that of PBS group (P 0.05). The results of H-FABP in each group were lower than 70 PG / mL. The results showed that the nucleic acid vaccine p43 and p53 gene of Trichinella spiralis had a good protective effect against Trichinella spiralis. And the two nucleic acid vaccine did not damage the myocardium of mice.
【作者单位】：东北农业大学动物医学学院;
【基金】：“十一五”国家科技支撑计划重大项目(2010BAD04B01) 国家自然科学基金资助项目(31172312)
【分类号】：R392
【正文快照】： 旋毛虫病是由旋毛虫引起的一种重要的人兽共患食源性寄生虫病,在全球范围内具有较高的感染率[1]。旋毛虫的抗原种类很多,成分非常复杂,主要分为虫体抗原、表面抗原、排泄分泌(ES)抗原及杆细胞颗粒相关抗原[2]。旋毛虫抗原中,ES抗原的保护作用最好,是制备免疫原的理想材料。由

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