艾迪注射液联合紫杉醇化疗对兔舌VX-2鳞癌模型调节性T细胞的影响

发布时间：2018-01-30 03:03

本文关键词： 兔舌VX-2鳞癌艾迪注射液紫杉醇注射液联合化疗调节性T细胞　出处：《遵义医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨艾迪注射液联合紫杉醇注射液化疗对兔舌VX-2鳞癌模型中调节性T细胞及其相关细胞因子IL-10、IL-35、TGF-β的影响。方法:建立48只兔舌VX-2鳞癌模型,随机分成4组,再选择健康兔24只,随机分成2组,共6组:A组(健康兔+生理盐水)、B组(健康兔+艾迪注射液)、C组(荷瘤兔+生理盐水)、D组(荷瘤兔+紫杉醇注射液)、E组(荷瘤兔+艾迪注射液)、F组(荷瘤兔+紫杉醇注射液+艾迪注射液)。建模后第15天开始分组用药,均通过兔耳缘静脉给药,在建模后第14天(用药前1天)、第22天、第29天均采集兔外周血,第22天各组分别随机处死6只实验兔并收集标本,第29天各组分别处死剩下的6只实验兔并收集标本。用HE染色法观察荷瘤兔舌癌癌灶的组织学形态,FCM检测各组实验兔外周血、颈淋巴结、舌癌原发灶、癌周组织的CD4+CD25+CD127low Treg/CD4+T细胞比例,ELISA检测血清的IL-10、IL-35、TGF-β浓度。结果:(1)B组的外周血、颈淋巴结Treg细胞比例以及血清的IL-10、IL-35、TGF-β浓度在用药前、用药后1周、2周与A组相比均无统计学差异(P0.05)。(2)C、D、E、F与A组比较:用药前,仅外周血的Treg细胞比例和血清的IL-35浓度,C、D、E、F均高于A组(P0.05)。用药后1周,外周血的Treg细胞比例和血清的IL-35、TGF-β浓度,C、D、E、F均高于A组(P0.05),而颈淋巴结的Treg细胞比例,仅C、D两组高于A组(P0.05),血清的IL-10浓度,仅C、D、E三组高于A组(P0.05)。用药后2周,外周血的Treg细胞比例和血清的IL-35浓度,C、D、E、F均高于A组(P0.05),而颈淋巴结的Treg细胞比例和血清的TGF-β浓度,仅C、D、E三组高于A组(P0.05),血清的IL-10浓度,仅C、D两组高于A组(P0.05)。(3)C、D、E、F四组间两两比较:用药后1周,荷瘤兔外周血、颈淋巴结的Treg细胞比例以及血清的IL-10、IL-35、TGF-β的浓度,D、E、F三组均低于C组(P0.05),D、E、F三组间两两相比均无统计学差异(P0.05)。用药后2周,D组与C组比较,仅血清IL-10浓度低于C组(P0.05);E组与C组比较,除舌癌原发灶的Treg细胞比例与C组无统计学差异(P0.05),其余指标均低于C组(P0.05),与D组相比,仅颈淋巴结的Treg细胞比例和血清IL-10浓度低于D组(P0.05);F组与C组比较,所有指标均低于C组(P0.05),与D组比较,除舌癌原发灶的Treg细胞比例与D组无统计学差异(P0.05),其余指标均低于D组(P0.05),与E组比较,仅血清TGF-β的浓度低于E组(P0.05)。(4)各组颈淋巴结、舌癌原发灶、癌周组织的Treg细胞比例在用药后1周(T1)与用药后2周(T2)组内比较:颈淋巴结与癌周组织仅C、D两组T2高于T1(P0.05),舌癌原发灶C、D、E、F四组T1与T2均无统计学差异(P0.05)。(5)实验兔血清的IL-10、IL-35、TGF-β浓度均与外周血的Treg细胞比例呈正相关(0.5r1,P0.01)。结论:艾迪注射液联合紫杉醇注射液化疗可抑制兔舌VX-2鳞癌模型中外周血、舌癌原发灶、舌癌癌周组织、颈淋巴结的CD4+CD25+CD127low Treg/CD4+T细胞比例及血清内IL-10、IL-35、TGF-β浓度的增高。
[Abstract]:Objective: to investigate the effects of Aidi injection combined with paclitaxel injection chemotherapy on regulatory T cells and their related cytokines IL-10 IL-35 in rabbit tongue VX-2 squamous cell carcinoma model. Methods: 48 rabbit tongue VX-2 squamous cell carcinoma models were established and randomly divided into 4 groups. 24 healthy rabbits were randomly divided into 2 groups. Group B (Aidi injection) group C (normal saline group D) (paclitaxel injection group E (Adi injection rabbit)). Group F (paclitaxel injection Aidi injection) was divided into groups on the 15th day after modeling and was administered via the rabbit ear vein on the 14th day (one day before, 22 days before the use of the drug). On the 29th day, the peripheral blood was collected. On the 22nd day, 6 experimental rabbits were randomly killed and the specimens were collected. On the 29th day, the remaining 6 experimental rabbits were killed and the specimens were collected. The histological morphology of tongue cancer foci was observed by HE staining and the peripheral blood and cervical lymph nodes were detected by FCM in each group. The ratio of CD4 CD25 CD127low Treg/CD4 T cells in peripheral tissues of tongue cancer was detected by Elisa. Results the percentage of Treg cells in peripheral blood, cervical lymph nodes and serum IL-10, IL-35 and TGF- 尾 concentration in group B were 1 week before and 1 week after administration of TGF- 尾. There was no statistical difference between group A and group A in 2 weeks. Compared with group A, only the proportion of Treg cells in peripheral blood and the concentration of IL-35 in serum were compared with those in group A. The percentage of Treg cells in peripheral blood and the concentration of IL-35 TGF- 尾 in peripheral blood were higher than those in group A (P 0.05). The percentage of Treg cells in cervical lymph nodes was higher in group C and D than in group A, and the concentration of serum IL-10 in group C was higher than that in group A. The ratio of Treg cells in peripheral blood and the concentration of IL-35 in serum in group E were higher than those in group A (P 0.05). However, the percentage of Treg cells and the concentration of TGF- 尾 in the cervical lymph nodes were higher in group C than in group A, and the concentration of IL-10 in serum was only C. Comparison of Treg cells in peripheral blood, cervical lymph nodes and serum IL-10 of rabbits with tumor one week after treatment. The concentration of TGF- 尾 in group C was lower than that in group C (P 0.05). There was no significant difference in the concentration of TGF- 尾 between group C and group C (P 0.05). 2 weeks after treatment, there was no significant difference in the concentration of TGF- 尾 between group C and group C (P 0.05). Compared with group C, serum IL-10 concentration in group D was lower than that in group C (P 0.05). Compared with group C, the percentage of Treg cells in group E was not significantly different from that in group C (P 0.05), and the other indexes were lower than those in group C (P 0.05). The proportion of Treg cells and the concentration of serum IL-10 in cervical lymph nodes were lower than those in group D (P 0.05). Compared with group C, all the indexes in group F were lower than those in group C (P0.05A). Compared with group D, there was no significant difference in the proportion of Treg cells except primary focus of tongue cancer between group D and group D (P 0.05). Compared with group E, the serum TGF- 尾 concentration was lower than that in group E (P 0.05, P 0.05, P 0.05, P < 0.05), and the primary focus of tongue cancer in each group was lower than that in group E. The proportion of Treg cells in pericancerous tissue was higher than that in T1 / T1 group and T2 / T2 group: T2 was higher in cervical lymph node and pericancerous tissue than that in T1 + P0. 05). There was no significant difference in T1 and T2 between the four groups (P < 0.05). The concentration of TGF- 尾 was positively correlated with the proportion of Treg cells in peripheral blood. Conclusion: Aidi injection combined with paclitaxel injection chemotherapy can inhibit peripheral blood, primary focus of tongue cancer and tissue around carcinoma of tongue in rabbit tongue VX-2 squamous cell carcinoma model. The percentage of CD4 CD25 CD127low Treg/CD4 T cells in cervical lymph nodes and the concentration of IL-10, IL-35 and TGF- 尾 in serum were increased.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8;R-332

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