人芽囊原虫病原学检查方法比较及形态观察
发布时间:2018-02-05 20:13
本文关键词: 人芽囊原虫 检出率 检出密度 改良酸醚离心沉淀法 出处:《中国病原生物学杂志》2013年02期 论文类型:期刊论文
【摘要】:目的比较3种人芽囊原虫病原学检查方法的检出效果。方法收集腹泻组400例、健康对照组394例送检粪便标本,分别采用生理盐水涂片法、碘液染色法和改良酸醚离心沉淀法检查人芽囊原虫,并观察镜下形态。结果腹泻患者粪便标本3种方法阳性检出率粪便为11.50%、12.75%和20.75%;改良酸醚离心沉淀法检出率高于其他两种方法(χ2=15.804,P0.05),且该法检出密度亦明显高于其他两种方法(χ2=144.715,P0.05)。健康对照组改良酸醚离心沉淀法阳性检出率(1.52%)和检出密度最高。粪便中人芽囊原虫最常见为空泡型,其次为颗粒型。结论临床检测人芽囊原虫时,应根据实际需要和检测条件选取合适的检查方法。
[Abstract]:Objective to compare the effect of 3 kinds of detection of blastocystishominis pathogenic detection methods. Methods 400 cases of diarrhea were collected, 394 cases of healthy control groups from stool specimens were used saline smear, iodine staining method and the modified acid ether centrifugal sedimentation method examination of Blastocystis hominis, and observed microscopically. The results of diarrhea methods 3 stool specimens of patients with positive rate of stool was 11.50%, 12.75% and 20.75%; modified acid ether centrifugal sedimentation rate is higher than the other two methods (2=15.804, P0.05), and the detection density is obviously higher than the other two methods (2= 144.715, P0.05). Healthy control group modified acid ether centrifugal precipitation positive rate (1.52%) and the highest density of feces. The detection of Blastocystis hominis was the most common type of cavitation, followed by particle type. Conclusion the clinical detection of Blastocystis hominis, should be based on the actual needs and select appropriate detection conditions. Method.
【作者单位】: 广西医科大学第一附属医院检验科;广西医科大学寄生虫学教研室;
【分类号】:R382.9
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