建立SD大鼠胚胎-胎仔发育指标的背景数据

发布时间：2018-02-24 17:11

  本文关键词： 数据处理 发育指标 大鼠　出处：《中国药理学与毒理学杂志》2013年03期 　论文类型：期刊论文

【摘要】：目的总结本研究中心的SD大鼠胚胎-胎仔发育毒性试验各个指标的正常值范围,建立SD大鼠胚胎-胎仔发育指标的背景数据,为生殖毒理研究提供参考。方法从本研究中心近期的生殖毒性试验中选取多批SD大鼠胚胎-胎仔发育毒性试验,整理对照组孕鼠的胚胎发育和胎仔生长发育指标数据,包括孕鼠的窝平均黄体数、着床数、平均活胎数、活胎率、吸收胎率、死胎率,胎仔体重、胎盘重、身长、尾长、外观畸形、内脏和骨骼发育指标等,将数据进行统计,分别计算各个指标的均数和标准差。结果本研究总结了214只孕鼠和2734只活胎仔的胚胎-胎仔发育指标。本研究所总结的孕鼠年龄约为50～90日龄,孕鼠妊娠第0天(GD 0)体重为(229±20)g,妊娠第20天(GD 20)体重为(380±34)g,整个孕期体重增重较大,食量随孕期增长而逐渐增加。GD 20安乐死并实施解剖,孕鼠窝平均黄体数为(15.6±2.8)个,窝平均着床数为(13.6±3.7)个,窝平均活胎数为(12.8±3.7)只,总吸收胎数为177(177/2734),早期吸收胎数为175(175/2734),晚期吸收胎数为2(2/2734),死胎数为0(0/2734),活胎率为(93.96±9.54)%,吸收胎率为(6.04±9.54)%,早期吸收胎率为(5.98±9.40)%,晚期吸收胎率为(0.07±0.69)%,死胎率为(0.00±0.00)%。胎仔发育指标中,雌性胎仔体重为(3.751±0.325)g,雄性胎仔体重为(3.942±0.364)g,胎仔胎盘重为(0.558±0.112)g,胎仔身长(37.17±1.41)mm,尾长为(13.17±0.79)mm,胎仔性别比为雄性/雌性=1291/1443=0.9。活胎仔中,胎仔外观畸形率为(0.06±0.64)%;骨骼变异胎仔率为(4.22±13.24)%,骨骼异常胎仔率为(2.27±8.17)%;内脏变异胎仔率为(25.81±25.51)%,内脏异常胎仔率为(0.88±4.43)%。结论本中心的SD大鼠胚胎-胎仔发育毒性试验各个指标的改变均比较稳定,胎仔自发畸形率较低。
[Abstract]:Objective to summarize the range of normal values of SD rat embryo-fetal developmental toxicity test, and to establish the background data of SD rat embryonic fetal development index. Methods A number of batches of SD rat embryo-fetal developmental toxicity tests were selected from the recent reproductive toxicity tests in this research center to collate the data of embryonic development and fetal growth and development of pregnant rats in the control group. Including the average number of corpus luteum, the number of implantation sites, the average number of live births, the rate of live foetus, the rate of absorption, the rate of stillbirth, the body weight, placenta weight, body length, tail length, appearance deformity, visceral and bone development indicators of pregnant mice. Results the embryonic development indexes of 214 pregnant rats and 2734 live foetus were summarized. The gestational age was about 50 ~ 90 days. The body weight of pregnant rats was 229 卤20g on day 0 of gestation and 380 卤34g of GD20 on the 20th day of gestation. The body weight of the whole pregnancy increased greatly, and the food intake increased gradually with the increase of pregnancy. The mean number of corpus luteum of pregnant mice was 15.6 卤2.8), and the mean number of corpus luteum of pregnant mice was 15.6 卤2.8. The average number of nests was 13.6 卤3.7, and the average number of live foetus was 12.8 卤3.7). The total number of absorbed fetuses was 177v / 2734, the number of early absorbers was 175v / 175 / 2734, the number of late absorbers was 2 / 2 / 2734, the number of stillbirths was 0 / 2 / 2734, the living rate was 93.96 卤9.54, the rate of absorption was 6.04 卤9.54, the rate of early absorption was 5.98 卤9.40, the late absorptive rate was 0.07 卤0.69, the stillbirth rate was 0.00 卤0.000.The fetal growth index was: 0. 00 卤0. 000.The rate of fetal development was 0. 00 卤0. 000.The rate of absorption was 6. 04 卤9. 54, while the rate of early absorption was 5. 98 卤9. 40, the rate of late absorption was 0. 07 卤0. 69, and the stillbirth rate was 0. 00 卤0. The body weight of female foetus was 3.751 卤0.325 渭 g, that of male fetus was 3.942 卤0.364 g, that of placenta was 0.558 卤0.112g, that of foetus was 37.17 卤1.41g, and that of tail was 13.17 卤0.79nm. the sex ratio of male to female was 1291r14430.0.The sex ratio of male and female was 1291r14430.9.The body weight of female foetus was 3.75 卤0.325g, and that of male foetus was 3.942 卤0.364g, the placenta weight of foetus was 0.558 卤0.112g. The deformity rate of fetal appearance was 0.06 卤0.64, the abnormal fetal rate was 2.27 卤8.17, the abnormal fetal rate was 2.27 卤8.17, the abnormal fetal rate was 25.81 卤25.51 and the abnormal visceral fetal rate was 0.88 卤4.430.Conclusion the developmental toxicity test of SD rat embryo and fetus in this center is of significance. The changes are relatively stable. The rate of spontaneous malformation of fetus was lower.
【作者单位】： 北京昭衍新药研究中心股份有限公司;
【分类号】：R321
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本文编号：1531041