白藜芦醇对肠易激综合征模型小鼠脑-肠轴影响研究

发布时间：2018-02-27 02:14

本文关键词： 白藜芦醇肠易激综合征脑-肠轴肝郁脾虚小鼠　出处：《浙江中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的本研究拟通过白黎芦醇对IBS动物模型进行治疗,以观察动物焦虑、抑郁等肝郁情志表现是否能得到改善,并通过肠道动力和内脏敏感性检测,以观察白藜芦醇是否有可能成为治疗IBS的潜在安全有效的药物。方法1.建立慢急性联合应激肠易激综合征模型ICR小鼠,小鼠随机分成8组,每组12只。正常对照组、慢急性联合应激模型组、白藜芦醇作用组(分低、中、高三个不同剂量,分别为2.5、5、10 mg/kg,灌胃)及三组阳性药物对照组。通过糖水消耗实验、旷场实验、大理石掩埋实验和高架十字迷宫实验等检测小鼠抑郁、焦虑样行为。并检测腹壁撤退反射检测内脏敏感性、色素推进实验检测肠道动力。结果慢急性联合应激肠易激综合征小鼠模型随着时间的延长表现出显著的抑郁,高中低三个剂量的白藜芦醇均能改善其抑郁行为。IBS模型小鼠均表现出恐高的焦虑,高剂量组白藜芦醇可以减轻其焦虑行为。在大理石掩埋实验中,IBS小鼠模型均表现出焦虑,高中低三个剂量的白藜芦醇均可改善其焦虑症状。而在内脏敏感性方面,在肠道给予稍大量空气后显著升高,高中低三个剂量的白黎芦醇均可降低IBS模型小鼠的肠道高敏感性。在肠道动力方面,IBS模型小鼠表现出肠道动力的减弱,中高剂量白藜芦醇显著逆转肠道动力的减弱。说明IBS小鼠存在抑郁焦虑等情志变化,并伴有肠道高敏感性和肠道动力减慢,而白藜芦醇可以逆转上述这些变化。本研究为白藜芦醇作为脑肠双靶点作用的治疗IBS的安全有效的药物提供了实验依据。结论慢急性联合应激肠易激综合征模型小鼠存在焦虑、抑郁等情志变化,并有肠道敏感性增加、肠道动力减慢等表现;白藜芦醇能够逆转肠易激综合征小鼠焦虑抑郁等症状,并改善肠道高敏感和肠道动力变化。
[Abstract]:Objective to observe whether the expression of depression and anxiety in IBS animal model can be improved, and to detect intestinal motility and visceral sensitivity. To observe whether resveratrol might be a potentially safe and effective drug in the treatment of IBS. Methods 1. Establish a model of chronic acute stress irritable bowel syndrome (ICR) mice. The mice were randomly divided into 8 groups with 12 rats in each group. Chronic acute combined stress model group, resveratrol group (low, middle and high three different doses of 2.5 渭 g / kg, intragastric perfusion) and three groups of positive drug control group. Through sugar consumption test, open field test, Marble burying test and elevated cross maze test were used to detect depression and anxiety in mice, and abdominal wall retreating reflex to detect visceral sensitivity. Results the mice model of irritable bowel syndrome with chronic acute stress showed significant depression over time. Three doses of resveratrol could improve the depressive behavior. The IBS model mice showed the anxiety of fear of heights, the high dose group of resveratrol alleviated their anxiety behavior, and the mice model of IBS showed anxiety in marble burying experiment. High and low doses of resveratrol improved anxiety symptoms. In visceral sensitivity, it increased significantly after the gut was given a little more air. Three doses of high and low doses of silylitol could reduce the high sensitivity of intestinal tract in IBS model mice, and the intestinal motility of IBS model mice was decreased in terms of intestinal motility. Resveratrol significantly reversed the decrease of intestinal motility in IBS mice, indicating that depression, anxiety and other emotional changes were associated with intestinal hypersensitivity and decreased intestinal motility in IBS mice. Resveratrol can reverse these changes. This study provides an experimental basis for the safety and efficacy of resveratrol in the treatment of IBS. Conclusion there is anxiety in the model mice of irritable bowel syndrome induced by chronic acute stress. Resveratrol could reverse symptoms such as anxiety and depression in irritable bowel syndrome mice and improve intestinal hypersensitivity and intestinal motility.
【学位授予单位】：浙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

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