早年慢性应激对青春期病理性攻击大鼠空间学习记忆及海马脑源性神经营养因子、5-羟色胺的影响

发布时间：2018-03-02 15:50

本文选题：脑源性神经营养因子　切入点：血清素　出处：《第二军医大学学报》2013年10期 　论文类型：期刊论文

【摘要】：目的探索早年慢性应激对青春期病理性攻击大鼠空间学习记忆能力,及对海马脑源性神经营养因子(BDNF)、5-羟色胺(5-HT)水平的影响。方法将20只雄性、出生后21d的Sprague-Dawley大鼠分为实验组、对照组,每组各10只。实验组大鼠采用孤养、昼夜颠倒、非奖赏性挫败、预激惹刺激、居住-入侵攻击等多种早年持续性应激方法持续到青春期。然后采用居住-入侵攻击实验检测两组青春期大鼠的攻击性,水迷宫实验观察其空间学习记忆能力,运用免疫组化方法检测大鼠海马内BDNF和5-HT水平。结果 (1)水迷宫实验:实验组大鼠在总路程、穿越目标区域次数、逃避潜伏期3项指标上与对照组差异有统计学意义(P0.05),而中心区域路程/总路程比值与对照组比较差异无统计学意义(P0.05)。(2)免疫组化结果显示:与对照组比较,实验组大鼠海马内BDNF和5-HT免疫阳性神经元数量均减少(P0.05),BDNF和5-HT表达降低。结论 BDNF和5-HT可能参与了青春期病理性攻击大鼠海马空间学习记忆能力的调控,并在空间学习记忆方面起着重要调控作用。
[Abstract]:Objective to explore the effects of early chronic stress on spatial learning and memory ability and the level of brain-derived neurotrophic factor BDNF 5-hydroxytryptamine (5-HT) in rats with pathological attack in adolescence. Methods Twenty male Sprague-Dawley rats were divided into two groups: experimental group. Control group, 10 rats in each group. Rats in the experimental group were isolated, day and night reversed, non-reward frustration, preirritation, Habitation-invasion attack and other early sustainable stress methods lasted into adolescence. Then, the aggression of two groups of rats was tested by habitation-invasion attack experiment, and the spatial learning and memory ability was observed by water maze experiment. The levels of BDNF and 5-HT in the hippocampus of rats were detected by immunohistochemical method. Results the water maze experiment showed that the rats in the experimental group passed through the target area in the total distance and the number of times. The three indexes of escape latency were significantly different from those of the control group (P 0.05), but the ratio of distance to total distance in the central region was not significantly different from that of the control group (P 0.05). The immunohistochemical results showed that: compared with the control group, there was no significant difference in the ratio of distance to total distance between the two groups. The number of BDNF and 5-HT immunoreactive neurons in the hippocampus of the experimental group decreased significantly. Conclusion BDNF and 5-HT may be involved in the regulation of spatial learning and memory ability in the hippocampus of rats attacked by puberty. And in spatial learning and memory plays an important role in regulation.
【作者单位】：重庆医科大学附属第一医院心理卫生中心;重庆医科大学附属第一医院神经病学实验室;
【基金】：重庆市自然科学基金(CSTC,2011jjA10104) 重庆医科大学国家自然科学预研资助项目(NSFYY201111)~~
【分类号】：R363

【参考文献】