STAg联合IFN-γ佐剂滴鼻免疫小鼠诱导GALT和系统免疫应答及抵抗弓形虫感染的研究

发布时间：2018-03-06 22:33

本文选题：刚地弓形虫　切入点：滴鼻免疫　出处：《中国病原生物学杂志》2013年01期 　论文类型：期刊论文

【摘要】：目的观察弓形虫可溶性速殖子抗原(STAg)联合重组人干扰素γ(IFN-γ)佐剂滴鼻免疫BALB/c小鼠诱导的肠相关淋巴组织(GALT)和系统免疫应答及抗弓形虫感染能力。方法 BALB/c小鼠72只随机分为免疫组和对照组。免疫组用STAg 20μg/只+IFN-γ佐剂1 000U/只(溶于20μl PBS中)滴鼻免疫,对照组用等量PBS滴鼻。共滴鼻免疫2次,间隔14d。于末次滴鼻后第14d两组各处死6只小鼠,取血并收集肠冲洗液,ELISA法测定IgG、IgA抗体;制备脾淋巴细胞、PP结淋巴细胞和肠上皮内淋巴细胞(intestinal intraepithelial lymphocytes,IEL)悬液并计数。用4×104个速殖子/只灌胃攻击其余小鼠。攻虫后第30d处死,计数肝脏、脑内速殖子数。结果免疫组小鼠小肠冲洗液IgA和IgG水平均显著高于对照组(P0.05),血清IgG抗体水平高于对照组(P0.05)。免疫组小鼠肠黏膜诱导部位PP淋巴细胞和效应部位IEL及脾淋巴细胞与对照组比较显著增生(P0.05)。攻击后30d,免疫组小鼠存活率为83.33%,对照组为40.00%,差异有统计学意义(P0.05),与对照相比,免疫组小鼠肝、脑内速殖子数分别减少86.62%和86.83%(P0.05)。结论 STAg联合IFN-γ滴鼻免疫BALB/c小鼠能诱导GALT和系统免疫应答,从而抵抗弓形虫感染。
[Abstract]:Objective To observe the effect of Toxoplasma gondii soluble tachyzoite antigen (STAg) combined with recombinant human interferon gamma (IFN- gamma) of gut associated lymphoid tissue induced by adjuvant intranasal immunization of BALB/c mice (GALT) infection and systemic immune response ability and anti Toxoplasma. Methods 72 BALB/c mice were randomly divided into immune group and control group. The immune group STAg 20 g/ +IFN- 1 000U/ gamma adjuvant (dissolved in 20 l PBS) intranasal immunization, the control group with the same amount of PBS nasal intranasal immunization. A total of 2 times, the last time interval 14D. after intranasal 14d two groups of 6 mice were killed, blood collection and intestinal washes determination of IgG, ELISA, IgA antibody; preparation of spleen lymphocytes, PP lymphocytes and intestinal intraepithelial lymphocytes (intestinal intraepithelial, lymphocytes, IEL) suspension and counting. Attack the remaining mice with 4 x 104 tachyzoites orally. Worm attack 30d after death, count liver, brain tachyzoite the number of sub immunized group. Intestinal washes of mice IgA and IgG were significantly higher than those in control group (P0.05), serum antibody level of IgG is higher than that of control group (P0.05). Mice intestinal immunity induced by mucosal sites of PP lymphocytes and the effect site IEL and spleen lymphocytes were proliferated significantly compared with the control group (P0.05). After the attack of 30d, the survival rate of immunized mice 83.33%, the control group was 40%, the difference was statistically significant (P0.05), compared with the control group, immune mice liver, the number of tachyzoites in brain were reduced by 86.62% and 86.83% (P0.05). Conclusion STAg combined with IFN- gamma intranasal immunization of BALB/c mice can induce GALT and systemic immune response to Toxoplasma gondii infection resistance.

【作者单位】：山西医科大学医学寄生虫学研究所寄生虫学教研室;山西医科大学第二临床医学院;
【基金】：国家自然科学基金项目(No.30640057) 山西医科大学2010年大学生创新项目基金项目
【分类号】：R392

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