白细胞介素1β对组织工程化椎间盘细胞基质金属蛋白酶13表达的作用

发布时间：2018-03-10 20:19

本文选题：白细胞介素β　切入点：基质金属蛋白酶　出处：《中国矫形外科杂志》2013年05期 　论文类型：期刊论文

【摘要】：[目的]观察不同浓度白细胞介素1β对组织工程化椎间盘细胞基质金属蛋白酶13表达的影响。[方法]将体外培养的兔组织工程化椎间盘细胞随机分为1、10、100μg/L浓度白细胞介素1β作用组及空白对照组,免疫组织化学检测基质金属蛋白酶13表达。[结果]1、10、100μg/L浓度白细胞介素1β组基质金属蛋白酶13阳性表达率均较对照组增高(P0.05)。随着白细胞介素1β浓度的增高,基质金属蛋白酶13阳性表达率显著升高,100μg/L浓度高于1、10μg/L浓度组(P0.05),10μg/L浓度组高于1μg/L浓度组(P0.05)。[结论]白细胞介素1β对兔组织工程化椎间盘细胞基质金属蛋白酶13表达的调控作用随其浓度的升高而呈正相关。
[Abstract]:[objective] to observe the effect of different concentrations of interleukin 1 尾 on the expression of matrix metalloproteinase 13 in tissue engineered intervertebral disc cells. [methods] cultured rabbit tissue engineered intervertebral disc cells were randomly divided into 100 渭 g / L of 100 渭 g / L fine white cells. IL-1 尾 group and blank control group, The expression of matrix metalloproteinase 13 was detected by immunohistochemistry. [results] the positive expression rate of matrix metalloproteinase 13 in 100 渭 g / L IL-1 尾 group was significantly higher than that in control group (P 0.05). The positive expression rate of matrix metalloproteinase 13 increased significantly. [conclusion] Interleukin-1 尾 can regulate the expression of matrix metalloproteinase 13 in tissue engineered intervertebral disc cells of rabbits with 100 渭 g / L concentration higher than 10 渭 g / L concentration group and 10 渭 g / L concentration group higher than 1 渭 g / L group. [conclusion] the effect of interleukin 1 尾 on matrix metalloproteinase 13 expression in rabbit tissue engineered intervertebral disc cells is significant. The control effect was positively correlated with the increase of its concentration.
【作者单位】：河南大学淮河医院骨科;
【基金】：河南省科技厅重点攻关项目(编号:112102310286)
【分类号】：R363

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