结核杆菌对巨噬细胞转铁蛋白受体和铁蛋白表达的影响

发布时间：2018-03-14 23:09

本文选题：结核分枝杆菌　切入点：巨噬细胞　出处：《中国细胞生物学学报》2013年05期 　论文类型：期刊论文

【摘要】：为了研究结核分枝杆菌国际标准强毒株H37Rv株和卡介苗菌株(BCG)分别感染巨噬细胞RAW264.7细胞株对细胞内转铁蛋白受体和铁蛋白表达量的时相变化及意义,分别于感染后1,6,12,18,24 h,应用ELISA和Western blot检测各组巨噬细胞中TfR和Fn的表达量。ELISA检测结果显示,各组感染巨噬细胞上清液中TfR表达量均高于对照组,在感染12,18 h差异最明显,具有统计学意义(P0.05)。Western blot检测结果显示:于模型建成后1,6,18 h各感染组差异有统计学意义(P0.05)。用ELISA和Western blot检测显示各组小鼠巨噬细胞内Fn随时间变化表达逐渐增强,在1 h时Fn的表达为对照组高于H37Rv组和BCG组;6 h后,感染组表达逐渐高于对照组,12 h时,H37Rv组对照组,差异具有统计学意义(P0.05),在18 h和24 h时,H37Rv组BCG组对照组,差异具有统计学意义(P0.05)。结核分枝杆菌感染导致巨噬细胞内TfR和Fn表达量增高,且与菌株毒力强弱有关,毒力强的菌株感染巨噬细胞的TfR和Fn的表达量更高。
[Abstract]:To study the temporal changes of transferrin receptor and ferritin expression in macrophage RAW264.7 cells infected with Mycobacterium tuberculosis strain H37Rv and Bacillus Calmette-Calmette Calmette Calmette Calmette Calmette-Guerin (BCG). The expression of TfR and FN in macrophages of each group was detected by ELISA and Western blot. The results of Elisa showed that the expression of TfR in the supernatant of infected macrophages was higher than that in control group, and the difference was most obvious at 1218 h after infection. The results showed that there were significant differences in the expression of FN with time by ELISA and Western blot in the macrophages of each group at 1: 6 ~ 6 h after the establishment of the model, and the expression of FN in the macrophages of each group was gradually increased with the development of Western blot, and the expression of FN in macrophages of each group was gradually increased with time. At 1 h, the expression of FN in the control group was higher than that in the H37Rv group and in the BCG group at 6 h, the expression of FN in the infected group was gradually higher than that in the control group at 12 h, the difference was statistically significant (P 0.05), and at 18 h and 24 h, the expression of FN in the H37Rv group was significantly higher than that in the H37Rv group at 18 h and 24 h. The expression of TfR and FN in macrophages was increased due to the infection of Mycobacterium tuberculosis, which was related to the virulence of the strains. The expression of TfR and FN in macrophages infected by the virulent strains was higher than that of the strains infected with Mycobacterium tuberculosis.
【作者单位】：石河子大学医学院病理生理学教研室/石河子大学新疆地方与民族高发病教育部重点实验室;
【基金】：国家自然科学基金(批准号:81260261、81160192、30960355、81260241) 新疆生产建设兵团医药专项资金(批准号:2012BA022) 石河子大学科学技术研究发展计划“自然科学与计划创新”重点项目(批准号:ZRKX2010ZD01)资助的课题~~
【分类号】：R378

【相似文献】