泛耐药铜绿假单胞菌氨基糖苷类修饰酶与16SrRNA甲基化酶基因检测研究

发布时间：2018-03-24 00:27

  本文选题：铜绿假单胞菌　切入点：氨基糖苷类　出处：《中华医院感染学杂志》2013年06期

【摘要】：目的研究泛耐药铜绿假单胞菌对氨基糖苷类的耐药机制,以指导临床合理使用抗菌药物,降低细菌耐药性的产生。方法收集2010年1-12月浙江绍兴第二医院住院患者痰液标本中分离到的泛耐药铜绿假单胞菌共20株,用聚合酶链反应(PCR)及序列分析的方法分析14种氨基糖苷类修饰酶基因与7种16SrRNA甲基化酶基因。结果 20株铜绿假单胞菌均检测到aac(6′)-Ⅱ及rmtB基因,检出率100.0%;17株检测到aac(6′)-Ⅰb,检出率85.0%;18株检测到ant(2″)-Ⅰ,检出率90.0%;2株检测到ant(3″)-Ⅰ,检出率10.0%。结论 aac(6′)-Ⅱ型氨基糖苷类修饰酶与rmtB型16SrRNA甲基化酶基因流行是泛耐药铜绿假单胞菌对氨基糖苷类耐药的重要原因。
[Abstract]:Objective to study the resistance mechanism of pan-resistant Pseudomonas aeruginosa to aminoglycosides and to guide the rational use of antimicrobial agents in clinic. Methods A total of 20 strains of pan-resistant Pseudomonas aeruginosa were isolated from sputum samples of hospitalized patients in Shaoxing Hospital of Zhejiang Province from January to December 2010. 14 aminoglycoside modified enzyme genes and 7 16SrRNA methylase genes were analyzed by polymerase chain reaction (PCR) and sequence analysis. The detectable rate was 100.0%. The detectable rate was 85.0%. The detection rate was 85.0%. The detectable rate was 90.0%. The detection rate was 90.0%. The detection rate was 90.010%, and the detection rate was 90.0%, and the detection rate was 85.0% and 85.0% respectively, and the detection rate was 90.0%. Conclusion the prevalence of aminoglycoside modifying enzyme type 鈪，

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