HSD1蛋白在精子发生过程中的空间特异性表达

发布时间：2018-03-25 20:11

本文选题：HSD　切入点：精子发生　出处：《基础医学与临床》2013年03期

【摘要】：目的研究HSD1蛋白在各级生精细胞中的空间特异性表达及其在细胞中的核质穿梭特性。方法分离小鼠生精细胞,用免疫荧光法观察HSD1蛋白的内源定位。构建pEGFP-C1-HSD1融合表达质粒并转染CHO细胞,用激光共聚焦和电镜观察HSD1融合蛋白的定位及其核质分布情况。结果 HSD1蛋白在初级精母细胞和圆形精子细胞的胞质中表达;在成熟精子细胞中主要表达于顶体和尾部。HSD1蛋白在CHO细胞中存在3种分布形式:细胞核型、细胞质型和核质分布型。进一步研究发现该蛋白在细胞中呈动态分布,存在细胞核-质间的穿梭特性。结论HSD1蛋白在生精细胞中呈空间特异性表达并且具有核质穿梭能力。
[Abstract]:Objective to study the spatial specific expression of HSD1 protein in spermatogenic cells and its nuclear and cytoplasmic shuttle characteristics. Methods Mouse spermatogenic cells were isolated. The endogenous localization of HSD1 protein was observed by immunofluorescence method. PEGFP-C1-HSD1 fusion expression plasmid was constructed and transfected into CHO cells. The localization and nuclear cytoplasmic distribution of HSD1 fusion protein were observed by laser confocal and electron microscopy. Results HSD1 protein was expressed in the cytoplasm of primary spermatocytes and round spermatocytes. In mature spermatozoa, the HSD1 protein is mainly expressed in acrosome and tail. There are three distribution forms of HSD1 protein in CHO cells: nuclear type, cytoplasmic type and cytoplasmic type. Conclusion the expression of HSD1 protein in spermatogenic cells is spatially specific and has the ability of nuclear and cytoplasmic shuttle.
【作者单位】：中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室生物化学与分子生物学系;
【基金】：国家科技支撑计划项目(2012BAI31B08) 国家重点实验室专项基金(2060204)
【分类号】：R363

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