解剖部位差异对脂肪干细胞生物学行为及成骨分化能力的影响

发布时间：2018-04-03 11:11

本文选题：脂肪干细胞　切入点：解剖部位　出处：《华中科技大学学报(医学版)》2013年05期

【摘要】：目的探讨不同解剖部位来源的脂肪干细胞在增殖、活性、成骨分化能力上的差异。方法实验所用脂肪均取自3只雌性新西兰大白兔脂肪组织,按解剖部位分组,分别为:腹股沟组、颈背部组、肾周组。各组脂肪经体外分离获得脂肪干细胞,并对其细胞增殖、活性、成骨分化能力进行检测。结果在MTT细胞增殖实验中,腹股沟组和颈背部组细胞增殖能力均明显优于肾周组(P0.05),而腹股沟组和颈背部组间无差异(P0.05);在活/死细胞染色检测细胞活性实验中,3组间细胞活性差异无统计学意义(P0.05);在半定量分析碱性磷酸酶(ALP)活性及qRT-PCR检测Runx2、COLL-1、OC表达以反映成骨分化能力的实验中,来源于皮下脂肪的两组(腹股沟组和颈背部组)的ALP活性高于肾周组(P0.05),并且腹股沟组比颈背部组的成骨能力更强(P0.05)。结论来源于皮下脂肪的脂肪干细胞具有更好的增殖和分化能力,其中腹股沟皮下脂肪来源的脂肪干细胞的性能更加优越。
[Abstract]:Objective to investigate the difference of proliferation, activity and osteogenic differentiation of adipose stem cells from different anatomical sites.Methods the adipose tissue of 3 female New Zealand rabbits were divided into three groups: inguinal group, cervical dorsal group and perirenal group.Adipose stem cells were isolated from adipose cells in vitro, and the proliferation, activity and osteogenic differentiation of adipose stem cells were measured.Results in MTT cell proliferation assay,The proliferative ability of groin group and cervical dorsal group were significantly better than that of perirenal group, but there was no difference between inguinal group and cervical back group.In the experiment of semi-quantitative analysis of alkaline phosphatase (ALP) activity and detection of Runx2COLL-1OC expression by qRT-PCR to reflect the osteogenic differentiation ability,Conclusion the adipose stem cells derived from subcutaneous adipose cells have better proliferation and differentiation ability, and the adipose stem cells derived from inguinal subcutaneous adipose cells have better performance.
【作者单位】：华中科技大学同济医学院附属同济医院泌尿外科;华中科技大学同济医学院附属同济医院骨科;
【基金】：国家自然科学基金资助项目(No.30700171,No.81270851) 华中科技大学同济医学院附属同济医院科研基金(2012)
【分类号】：R329.28

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