双轴旋转运动刺激对大鼠前边缘皮质神经元放电的影响

发布时间：2018-04-08 16:04

本文选题：前庭器官　切入点：扣带回　出处：《神经解剖学杂志》2013年06期

【摘要】：目的:探索前庭信息是否调节前边缘皮质(prelimbic cortex,PrL)区神经元的活动。方法:体重130~150 g雄性SD大鼠,分成两组:对照组和运动刺激组。运动刺激组动物接受2 h双轴旋转运动刺激,以刺激前庭感受器。采用全细胞脑片膜片钳技术,分别在电流钳和电压钳模式下,刺激PrL浅层(II/III层)并记录PrL深层(V/VI层)神经元,分析动作电位(action potential,AP)特性和诱发的兴奋性突触后电流(evoked excitatory postsynaptic current,eEPSC)。结果:2 h双轴旋转刺激没有改变PrL神经元AP阈值、幅值、半幅时程、峰值上升相最大斜率以及峰值下降相最大斜率等参数(P0.05,n=10);但向神经元输入波宽180 ms,80~280 pA的脉冲电流后,旋转刺激组PrL神经元放电数目显著增加(P0.05,n=8)。结论:前庭信息可影响PrL神经元活动,故PrL很可能是接受和处理前庭信息的高级脑结构之一。
[Abstract]:Aim: to investigate whether vestibular information regulates the activity of neurons in the prelimbic cortexus region of the prelimbic cortexus.Methods: 150 g male SD rats were divided into two groups: control group and motor stimulation group.The animals in the motor stimulation group were stimulated by biaxial rotation for 2 h to stimulate the vestibular receptors.Whole cell patch clamp technique was used to stimulate PrL superficial layer II / III) and record PrL deep layer V / VI) neurons in current clamp mode and voltage clamp mode, respectively. The action potential potential (APP) characteristics and evoked excitatory postsynaptic current evoked excitatory postsynaptic current EPSCN were analyzed.Results the PrL neuron AP threshold, amplitude, half-amplitude duration, maximum slope of peak ascending phase and maximum slope of peak descending phase were not changed by two-axis rotation stimulation for 2 h, but the pulse current of 180ms and 80280pA was injected to the neuron.The number of PrL neurons in rotational stimulation group increased significantly (P 0.05).Conclusion: vestibular information may affect the activity of PrL neurons, so PrL may be one of the advanced brain structures to receive and process vestibular information.
【作者单位】：第四军医大学人体解剖与组织胚胎学教研室暨梁挾琚脑研究中心;西安市公安局霸桥分局刑警大队;
【基金】：国家自然科学基金(81071105;81171279)
【分类号】：R338

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