硼替佐米对K562细胞增殖、凋亡及SHIP基因表达的影响

发布时间：2018-04-29 10:48

本文选题：硼替佐米 + K细胞　；参考：《中国实验血液学杂志》2013年04期

【摘要】：本研究旨在探讨蛋白酶体抑制剂硼替佐米(bortezomib)对K562细胞的增殖、凋亡及SHIP基因表达的影响。将不同浓度的硼替佐米作用于K562细胞,采用MTT法测定细胞增殖活性,用流式细胞术检测细胞凋亡,RT-PCR方法检测SHIP mRNA表达。结果表明,硼替佐米10、20、50和100 nmol/L作用于K562细胞24 h,细胞增殖抑制率分别为(5.76±1.47)%、(10.55±1.59)%、(17.14±2.05)%和(27.69±3.57)%,空白对照组为(1.30±0.10)%;20nmol/L硼替佐米作用于K562细胞24、48、72 h,细胞增殖抑制率分别为(10.55±1.59)%、(16.33±2.53)%、(19.78±1.56)%,24 h组与48 h组比较,差异具有统计学意义(P0.05),10、20、50、100 nmol/L硼替佐米作用于K562细胞24h,Annexin V-FITC/PI双标法显示其凋亡率分别为(12.7±0.6)%、(26.9±0.9)%、(32.6±1.2)%、(72.5±1.5)%,均高于对照组(1.0±0.5)%(P0.05)。RT-PCR法检测结果显示应用硼替佐米作用后SHIP mRNA表达上调明显,与空白对照组比较差异有统计学意义。结论:硼替佐米呈时间、浓度依赖性抑制K562细胞增殖,并能通过上调SHIP基因的表达诱导细胞凋亡。
[Abstract]:The aim of this study was to investigate the effects of bortezomibb, a proteasome inhibitor, on the proliferation, apoptosis and SHIP gene expression of K562 cells. The proliferation activity of K562 cells was determined by MTT assay, and the expression of SHIP mRNA was detected by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the inhibition rates of proliferation of K562 cells were 5.76 卤1.47% and 17.14 卤2.05% and 27.69 卤3.57%, respectively, when bortezomil 1020 and 100 nmol/L were applied to K562 cells for 24 h and 100 nmol/L, respectively, while those in the blank control group were 1.30 卤0.1020 nmol / L borizomil for 24448 hours, and the inhibition rates of cell proliferation were 10.55 卤1.59m and 16.33 卤2.53, 19.78 卤1.56 and 19.78 卤1.56g / h, respectively. The difference was statistically significant (P 0.05, P 0. 05, P 0. 05, 10, 20, 50, 100 nmol/L bortezomil) treated K562 cells for 24 h, Annexin V-FITC/PI double labeling method showed that the apoptotic rate was 26. 9 卤0. 9 卤0. 9%. The expression of SHIP mRNA in K562 cells was significantly higher than that in the control group (1. 0 卤1. 5 卤1. 5 卤1. 5 卤1. 5). The results showed that the expression of SHIP mRNA was up-regulated after treatment with bortezomil. Compared with the blank control group, the difference was statistically significant. Conclusion: bortezomil can inhibit the proliferation of K562 cells in a dose-dependent manner and induce apoptosis by up-regulating the expression of SHIP gene.
【作者单位】：保定市第一医院血液科;
【分类号】：R363

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