LPS对大鼠星形胶质细胞HSPA12B表达的影响

发布时间：2018-05-07 01:05

本文选题：脂多糖 + 星形胶质细胞　；参考：《中国免疫学杂志》2013年10期

【摘要】：目的:探讨内毒素脂多糖(Lipopolysaccharide,LPS)对大鼠星形胶质细胞热休克蛋白A12B(Heat shock proteins A 12B,HSPA12B)表达变化和细胞定位的影响。方法:用不同浓度(0.01、0.1、1、10μg/ml)和同一浓度(1μg/ml)不同时间(1、2、4、6、8、12、24小时)的LPS刺激星形胶质细胞,分别用RT-PCR和Western blot法检测星形胶质细胞HSPA12B的mRNA和蛋白质水平表达情况,间接免疫荧光双标法检测HSPA12B在星形胶质细胞中的细胞定位。结果:用LPS 1μg/ml刺激1小时后,HSPA12B mRNA的表达增加,4小时表达活性最高。HSPA12B蛋白水平表达变化与mRNA水平变化相一致。免疫细胞化学证明LPS诱导星形胶质细胞HSPA12B的表达定位在胞核。结论:LPS可诱导星形胶质细胞HSPA12B表达上调。HSPA12B可作为一种炎症反应蛋白参与炎症反应过程。
[Abstract]:Aim: to investigate the effect of lipopolysaccharide (LPS) on the expression of heat shock protein (A12B(Heat shock proteins A 12B) and its cellular localization in rat astrocytes. Methods: the astrocytes were stimulated with LPS at different concentrations of 0. 01 ~ 0. 1 ~ 1 ~ (1 渭 g / ml) and 1 渭 g / ml of the same concentration of 1 渭 g / ml). The expression of mRNA and protein in astrocytes was detected by RT-PCR and Western blot methods. The localization of HSPA12B in astrocytes was detected by indirect immunofluorescence double labeling method. Results: after 1 hour stimulation with LPS 1 渭 g/ml, the expression of HSPA12B mRNA was increased. The expression of HSPA12B protein was the highest at 4 hours. The change of HSPA12B protein level was consistent with the change of mRNA level. Immunocytochemistry showed that the expression of HSPA12B in astrocytes induced by LPS was localized in the nucleus. Conclusion the up-regulation of HSPA12B expression and HSPA12B may be involved in the process of inflammation as a kind of inflammatory response protein.
【作者单位】：浙江省绍兴市人民医院;浙江大学绍兴医院输血科;南通大学基础医学院免疫与微生物实验室;
【分类号】：R363

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