甘草酸苷体外上调乙肝疫苗无应答者免疫功能

发布时间：2018-05-10 19:05

本文选题：乙肝疫苗 + 无应答　；参考：《中国免疫学杂志》2013年07期

【摘要】：目的:研究甘草酸苷(GL)对乙肝疫苗无应答者免疫功能的影响,为使乙肝疫苗应答率得到有效提升提供一种可行的方法。方法:分离研究对象PBMC,加入HBsAg和(或)GL的条件下体外培养,Real-time PCR测定刺激后IFN-γ、IL-4、IL-10、CD80、CD86 mRNA的表达水平,并与对照组和应答组比较。收集非贴壁细胞用于培养CTL,贴壁细胞诱导分化为DC;CCK-8法检测各组DC刺激同种异体淋巴细胞增殖的能力及DC激活的CTL细胞对K562细胞的杀伤作用。结果:HBsAg+GL联合刺激组PBMC的IFN-γ、IL-10、CD80、CD86 mRNA表达水平高于HBsAg单独刺激组,差异均有统计学意义(P0.05),但IFN-γmRNA表达低于应答组(P0.05),IL-10、CD80 mRNA无明显差异(P0.05),而CD86 mRNA表达高于应答组(P0.05);IL-4 mRNA表达无明显变化;与HBsAg单独刺激组比较,HBsAg+GL联合刺激组DC刺激同种异体淋巴细胞增殖的作用明显增强(P0.05),且高于应答组(P0.05),其激活的CTL对K562细胞杀伤作用亦显著提高(P0.05),接近应答组水平(P0.05)。结论:GL对乙肝疫苗无应答者免疫功能具有调节作用,联合HBsAg有可能诱导机体创造更有利于乙肝保护性抗体产生的环境,为其临床应用提供实验依据。
[Abstract]:Aim: to study the effect of glycyrrhizin GLN on immune function of hepatitis B vaccine non-responders and to provide a feasible method to improve the response rate of hepatitis B vaccine. Methods: PBMCs were isolated and cultured in vitro with HBsAg and (or) GL to determine the expression of IL-10, IL-10, CD80 and CD86 mRNA in IFN- 纬 -IL-4, IL-10 and IL-10, and compared with those in control group and response group. Non-adherent cells were collected to culture CTLs. The ability of DC to stimulate the proliferation of allogeneic lymphocytes and the cytotoxicity of DC activated CTL cells to K562 cells were detected by DC-CCK-8 method. Results the expression level of IFN- 纬 -IL-10 IL-10 CD80 ~ + CD86 mRNA in PBMC group was higher than that in HBsAg alone group (P 0.05), but the expression of IFN- 纬 mRNA was lower than that of P0.05 + IL-10 + CD80 mRNA in response group (P 0.05), but the expression of CD86 mRNA was higher than that of response group (P 0.05). There was no significant change in the expression of IL-4 mRNA. Compared with the HBsAg alone stimulation group, the proliferation of allogeneic lymphocytes stimulated by DC in combination with HBsAg GL was significantly increased than that in the response group. The cytotoxicity of activated CTL to K562 cells was also significantly increased, which was close to the level of P0.05 in the response group. Conclusion the immune function of non-responders with hepatitis B vaccine can be regulated by WJ GL, and combined with HBsAg can induce the body to create a more favorable environment for the production of hepatitis B protective antibody, and provide the experimental basis for its clinical application.
【作者单位】：暨南大学第二临床医学院深圳市人民医院检验科;香港大学深圳医院病理科;
【基金】：广东省自然科学基金(07300964)资助
【分类号】：R392.1

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