巨噬细胞吞噬黑素化与非黑素化马尔尼菲青霉的电镜观察

发布时间：2018-05-12 12:30

本文选题：马尔尼菲青霉 + 巨噬细胞　；参考：《中国人兽共患病学报》2013年06期

【摘要】：目的通过透射电镜(TEM)比较巨噬细胞吞噬黑素化与非黑素化马尔尼菲青霉酵母相孢子的区别,初步探讨黑素在马尔尼菲青霉致病机制中所发挥的作用。方法将马尔尼菲青霉酵母相孢子与Ana-1小鼠巨噬细胞系共同培养,分为处理组A、B、C和D组:A组加入干扰素-γ及L-DOPA,B组仅加入干扰素-γ,C组仅加入L-DOPA,D组不加干扰素-γ及L-DOPA,设置基础对照组E为加入L-DOPA培养的巨噬细胞。7d后离心收集各组巨噬细胞和孢子,透射电镜观察并比较其超微形态变化。结果无论是否加入干扰素-γ均可观察到巨噬细胞吞噬马尔尼菲青霉酵母相孢子现象。加入L-DOPA培养的A、C组巨噬细胞内外的酵母细胞壁内侧面均产生了颗粒状电子致密物,一个巨噬细胞吞噬孢子数目为2～6个。未加入L-DOPA培养的B、D组巨噬细胞内外的酵母细胞壁内侧面无颗粒状电子致密物,一个巨噬细胞吞噬孢子数目为10～25个。结论 L-DOPA可以促进马尔尼菲青霉酵母相孢子黑素颗粒的合成,黑素化培养的马尔尼菲青霉酵母相孢子逃避巨噬细胞吞噬的能力增强。
[Abstract]:Objective to investigate the role of melanin in the pathogenesis of Penicillium marneffei by comparing the phagocytosis of macrophages with that of non-melanocytic Penicillium marneffei by transmission electron microscopy (TEM). Methods the yeast spores of Penicillium marneffei and Ana-1 mouse macrophage cell lines were co-cultured. They were divided into two groups: group A, and group D, group A: group A added interferon- 纬 and L-DOPA-B, group C added only interferon- 纬 and L-DOPA, group C only added L-DOPA-D without interferon- 纬 and L-DOPA. After 7 days, macrophages and spores of each group were collected by centrifugation in group E as macrophages cultured with L-DOPA. The ultrastructural changes were observed and compared by transmission electron microscope (TEM). Results the phagocytosis of Penicillium marneffei yeast spores by macrophages was observed whether or not interferon-纬 was added. Granular electron density was produced in the inner side of the cell wall of the macrophages in group A and C cultured with L-DOPA, and the number of phagocytic spores of one macrophage ranged from 2 to 6. There were no granular electron densification in the inside and outside of the cell wall of the macrophages in group B ~ D without L-DOPA. The number of phagocytic spores of one macrophage ranged from 10 to 25. Conclusion L-DOPA can promote the synthesis of spore melanin granules in Penicillium marneffei, and the ability of the spores of Penicillium marneffei cultured to escape phagocytosis of macrophages is enhanced.
【作者单位】：广西医科大学第一附属医院皮肤性病科;
【基金】：国家自然科学基金项目(No.81160191) 广西自然科学基金项目(No.2011GXNsFA018255)联合资助~~
【分类号】：R379

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