工业噪声性听力损失动物模型的构建与应用

发布时间：2018-05-18 12:50

本文选题：噪声性听力损失(NIHL) + 稳态噪声　；参考：《中国工业医学杂志》2013年05期

【摘要】：目的构建工业噪声性听力损失动物模型。方法将50只雄性豚鼠随机分为5组,除对照组外其余4组分别予以85 dB(SPL)、95 dB(SPL)、105 dB(SPL)和115 dB(SPL)的模拟稳态工业噪声暴露28 d(6 h/d),于暴露前及暴露后7 d分别进行听性脑干反应(ABR)检测,以评价各组豚鼠噪声暴露后的听阈位移水平,暴露后取豚鼠耳蜗观察毛细胞的病理学变化。结果对照组及各噪声暴露组豚鼠听阈位移水平变化均表现出明显不同,平均听阈位移水平对照组为0.00 dB、85 dB(SPL)暴露组为6.75 dB、95 dB(SPL)暴露组为13.25 dB、105 dB(SPL)暴露组为21.50 dB、115 dB(SPL)暴露组为28.00 dB,听阈位移水平变化随着噪声暴露强度的增强而增加,不同组间差别有统计学意义(F=319.995,P=0.00);病理学观察发现耳蜗毛细胞结构随着噪声暴露强度的增加而损伤程度加重。结论模拟工业噪声性听力损失的豚鼠模型构建成功,它将为职业噪声性听力损失的发生机制及干预提供良好的研究平台。
[Abstract]:Objective to establish an animal model of industrial noise-induced hearing loss. Methods Fifty male guinea pigs were randomly divided into 5 groups. The other four groups except the control group were treated with 85 dBL (95 dBN) and 115 dB (SPLL) respectively. The auditory brainstem response (ABR) was measured before and 7 days after exposure to simulated steady-state industrial noise (28 d ~ (6) h / d ~ (-1). To evaluate the threshold displacement of guinea pigs after noise exposure, the cochlea of guinea pigs were taken to observe the pathological changes of hair cells. Results the changes of auditory threshold displacement in the control group and the noise exposure group were significantly different. The average threshold displacement level in the control group was 6.75 dB ~ 95 dB ~ (-1) vs 13.25 dB ~ 105 dB ~ (-1) in the control group and 21.50 dB ~ (115) dB ~ (-1) in the control group was 28.00 dB ~ (-1), and the change of the threshold displacement level was increased with the increase of the noise exposure intensity, and the mean threshold displacement level in the control group was 6.75 dB ~ (+) 95 dB ~ (-1) / L ~ (-1) respectively. The difference between different groups was statistically significant, and the pathological observation showed that the structure of cochlear hair cells increased with the increase of noise exposure. Conclusion the guinea pig model of industrial noise-induced hearing loss is successfully constructed, which will provide a good research platform for the mechanism and intervention of occupational noise-induced hearing loss.
【作者单位】：广东药学院公共卫生学院;广州市职业病防治院;
【基金】：广东省大学生创新训练项目(编号:1057312041)
【分类号】：R135.8;R-332

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