单人袖套法建立小鼠颈部心脏移植模型

发布时间：2018-05-23 06:49

本文选题：心脏移植 + 模型　；参考：《上海医学》2013年05期

【摘要】：目的在双人袖套法建立小鼠颈部心脏移植模型的基础上,对其核心步骤进行改进,使手术由单人即可完成。方法供、受体分别为BALB/c小鼠(50只)和C57BL/6小鼠(50只),应用内芯固定套管的"袖套"法血管吻合技术,进行小鼠颈部心脏移植。术后每天触摸小鼠颈部观察移植心脏的搏动情况。移植心脏搏动持续3d以上即表示移植成功,观察移植心脏的存活时间。术后第7天随机选取10只受体小鼠将其处死,取其移植心脏,经10%多聚甲醛固定、石蜡包埋、切片后脱水、固定,经苏木精-伊红(H-E)染色,置于光学显微镜下观察移植心脏的心肌结构,判断受体小鼠移植心脏的排斥反应分级。结果正式实验50次。术后移植心脏复跳率为100%。1只受体小鼠术后未苏醒,另1只受体小鼠术后第2天移植心脏停跳;单人袖套法建立小鼠颈部心脏移植模型的成功率为96%(48/50)。供心缺血时间18～35min,平均供心缺血时间为(26.2±5.4)min;手术时间73～119min,平均手术时间为(93.5±12.7)min;移植心脏存活时间7～9d,平均移植心脏存活时间为(8.1±0.6)d。病理学检查示,移植心脏的心肌细胞间质有大量淋巴、巨噬细胞浸润,伴有明显的水肿、出血和心肌坏死。10只小鼠的移植心脏均出现排斥反应,其中3A级排斥反应2只,3B级5只,4级3只。结论经改进后的单人袖套法建立小鼠颈部心脏移植模型的成功率较高,且操作简便,值得推广。
[Abstract]:Objective on the basis of establishing mouse cervical heart transplantation model by double cuff method, the core steps were improved to make the operation complete by single person. Methods the recipients were BALB/c mice (n = 50) and C57BL/6 mice (n = 50). The cervical heart transplantation was carried out by using the technique of "cuff" vascular anastomosis with inner core fixation cannula. Every day after operation, the mouse neck was touched to observe the pulsatility of the transplanted heart. The beating duration of the transplanted heart for more than 3 days indicated that the transplant was successful, and the survival time of the transplanted heart was observed. On the 7th day after operation, 10 recipient mice were randomly selected to be killed, and their hearts were transplanted. The hearts were fixed with 10% paraformaldehyde, embedded in paraffin, dehydrated and fixed by sections, and stained by hematoxylin-eosin (H-E). The myocardial structure of the transplanted heart was observed under optical microscope, and the rejection grade of the transplanted heart in the recipient mice was determined. Results 50 formal experiments were conducted. After operation, 100.1 recipient mice did not wake up, the other one received cardiac arrest on the second day after operation, and the success rate of single sleeve method was 96 / 48 / 50. The mean ischemic time of donor heart was 26.2 卤5.4 min, the mean operation time was 73.5 卤12.7 min, the mean ischemic time of donor heart was 73.5 卤12.7 min, the mean time of donor heart ischemia was 8.1 卤0.6 min, the survival time of donor heart was 79 days, the mean survival time of donor heart was 8.1 卤0.6 minutes. Histopathological examination showed that there were a large number of lymphoid, macrophage infiltration, edema, hemorrhage and myocardial necrosis in the cardiac stroma of the transplanted heart in 10 mice. Among them, there were 3 cases of 3 A grade rejection, 3 cases of grade 3 B, 5 cases of grade 4 and 3 cases of grade 4 rejection. Conclusion the improved single cuff method has a high success rate and is worth popularizing because of its high success rate and simple operation.
【作者单位】：复旦大学附属中山医院心外科;
【基金】：上海市重点学科建设项目资助(B116)
【分类号】：R-332

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