细粒棘球蚴egG1Y162疫苗对小鼠免疫应答的研究

发布时间：2018-05-27 17:06

本文选题：细粒棘球蚴egGY疫苗 + 免疫应答　；参考：《中国人兽共患病学报》2013年03期

【摘要】：目的观察细粒棘球蚴egG1Y162疫苗免疫小鼠后机体的免疫应答状况。方法实验组、GST对照组、佐剂组和正常组小鼠分别注射egG1Y162疫苗、谷胱甘肽巯基转移酶(GSTs)、佐剂(FCA)和生理盐水(NS),在免疫第0、2、4、6、8和10周,收集各组血清用酶联免疫吸附法(ELISA法)检测抗体滴度,并检测各组血清抗体IgG的动态变化。第10周用四甲基偶氮唑蓝实验(MTT法)检测免疫小鼠脾淋巴细胞体外刺激后的增殖反应,用AnnexinV-FITC和碘化丙啶(PI)双染色法检测脾细胞凋亡率。结果 egG1Y162疫苗免疫组小鼠在第2周免疫后检测到抗体,在免疫第10周,抗体滴度可达到1∶25 600。血清抗体IgG在免疫第2～10周不断升高,并在免疫第10周达到最高水平。MTT法显示egG1Y162疫苗免疫组小鼠脾淋巴细胞在体外能被egG1Y162疫苗特异性刺激增生。疫苗刺激后疫苗组增殖水平显著高于对照组、佐剂组和正常组(P0.05)。AnnexinV-FITC和PI双染色法结果显示实验组小鼠脾细胞原液和ConA刺激细胞凋亡发生率均显著低于对照组(P0.01),每组ConA刺激脾细胞凋亡发生率显著高于相应的原液培养(P0.01)。结论细粒棘球绦虫egG1Y162疫苗可诱导小鼠产生高效价抗体并可刺激脾淋巴细胞增殖活化、抑制脾细胞凋亡,特异性抗体反应和脾淋巴细胞增殖活化促进机体产生体液免疫反应和细胞免疫反应,共同协调诱导机体产生免疫应答反应。
[Abstract]:Objective to observe the immune response of mice immunized with egG1Y162 vaccine. Methods the control group, adjuvant group and normal control group were immunized with egG1Y162 vaccine, glutathione mercaptotransferase (GSTS), adjuvant (FCA) and normal saline (NS) respectively at the 8th and 10th week after immunization. The antibody titers were detected by Elisa and the dynamic changes of serum antibody IgG were detected in each group by enzyme-linked immunosorbent assay (Elisa). In the 10th week, the proliferation response of splenic lymphocytes stimulated by tetramethylazolium in vitro was detected by MTT assay, and the apoptosis rate of splenocytes was detected by AnnexinV-FITC and Pi double staining. Results the antibody was detected in mice immunized with egG1Y162 vaccine at the second week, and the titer of antibody reached 1:25 600 at the 10th week. Serum antibody IgG increased continuously at the 2nd week of immunization and reached the highest level at the 10th week. The results showed that spleen lymphocytes of mice immunized with egG1Y162 vaccine could be specifically stimulated by egG1Y162 vaccine in vitro. The proliferative level of vaccine group was significantly higher than that of control group. The results of P0.05U, Annexin V-FITC and Pi double staining in adjuvant group and normal group showed that the incidence of splenocyte apoptosis in the experimental group was significantly lower than that in the control group (P 0.01), and the rate of splenocyte apoptosis stimulated by ConA in each group was significantly higher than that in the corresponding culture medium (P 0.01). Conclusion Echinococcus granulosus egG1Y162 vaccine can induce the production of high titer antibody, stimulate the proliferation and activation of spleen lymphocytes, and inhibit the apoptosis of spleen cells. Specific antibody reaction and splenic lymphocyte proliferation promote humoral immune response and cellular immune response, and coordinate the induction of immune response.
【作者单位】：新疆医科大学基础医学院;新疆医科大学第一附属医院省部共建国家重点实验室培育基地新疆重大疾病医学重点实验室;
【基金】：国家自然科学基金项目(No.31000411,No.30901374,No.81160378,No.81060135,No.81160200,No.81201303) 新疆自治区高校科研计划项目(No.XJEDU2010S25)~~
【分类号】：R392.12

【参考文献】