NS398对Aβ肽损伤原代培养海马神经元的影响

发布时间：2018-05-29 19:14

本文选题：细胞培养 + 海马神经元　；参考：《神经解剖学杂志》2013年04期

【摘要】：目的:探讨Cox-2抑制剂NS398对损伤神经元的保护作用。方法:将原代培养的SD大鼠海马神经元随机分为空白对照组、Aβ肽细胞模型组和NS398实验组。以10μmol/L Aβ25-35制作原代培养海马神经元损伤模型,以四氮唑溴盐(MTT)比色法检测细胞存活率,免疫细胞化学法检测Cox-2、Caspase-3的表达。结果:Aβ25-35细胞模型组神经元形态变化明显,细胞存活率明显下降,Cox-2和Caspase-3表达增强,与空白组比较有显著差异(P0.01)。NS398使神经元细胞存活率明显提高并使Cox-2和Caspase-3表达减弱,与Aβ肽模型组比较具有显著性差异(P0.01)。结论:Aβ25-35导致Cox-2和Caspase-3的表达增强,并使SD大鼠原代培养海马神经元变性、死亡。NS398可能通过抑制Cox-2和Caspase-3的表达保护原代培养海马神经元。
[Abstract]:Objective: to investigate the protective effect of Cox-2 inhibitor NS398 on injured neurons. Methods: primary cultured hippocampal neurons of SD rats were randomly divided into control group and NS398 group. The primary hippocampal neuronal injury model was made with 10 渭 mol/L A 尾 25-35. The survival rate of neurons was determined by MTT assay and the expression of Caspase-3 was detected by immunocytochemistry. Results the neuronal morphology and the expression of Cox-2 and Caspase-3 were significantly decreased in the cell model group. Compared with the control group, the expression of Cox-2 and Caspase-3 was significantly increased, and the expression of Cox-2 and Caspase-3 was decreased. Compared with A 尾 peptide model group, there was significant difference (P 0.01). Conclusion the expression of Cox-2 and Caspase-3 was increased by 尾 25-35, and the primary cultured hippocampal neurons in SD rats were denatured. NS398 may protect primary cultured hippocampal neurons by inhibiting the expression of Cox-2 and Caspase-3.
【作者单位】：海南医学院附属医院神经内科;海南医学院附属医院肿瘤外科;海南医学院附属医院海南省药物安全性评价研究中心;
【基金】：海南省教育厅高等学校科学研究资助项目(Hjkj2008-44)
【分类号】：R338

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