氟化钠诱导大鼠肾脏细胞DNA损伤及细胞凋亡的实验研究

发布时间：2018-05-30 02:23

本文选题：氟 + 大鼠肾细胞　；参考：《毒理学杂志》2013年06期

【摘要】：目的研究氟对大鼠肾脏细胞DNA损伤和细胞凋亡的影响。方法 48只SD大鼠随机分为4组(对照组、低氟组、中氟组和高氟组),每组12只,分别饮用含氟化钠为0、50、100和200 mg/L的去离子水,染毒120 d。动物处死后,用单细胞凝胶电泳技术(SCGE)检测氟化物对大鼠肾细胞DNA损伤,流式细胞术(FCM)检测细胞凋亡。结果彗星实验表明,各染氟组肾细胞拖尾率明显高于对照组(P0.01)。染氟组大鼠肾细胞DNA迁移长度均高于对照组,高氟组与对照组比较,差异有统计学意义(P0.01),中氟组与对照组比较,差异有统计学意义(P0.05)。流式细胞术结果表明低氟组肾细胞凋亡率较对照组显著增高(P0.05),中氟组、高氟组较对照组显著增高(P0.01)。大鼠肾细胞DNA损伤与细胞凋亡之间存在明显的正相关关系(r=0.959,P0.01)。结论氟可导致大鼠肾细胞DNA损伤,诱导肾细胞凋亡,氟诱导的大鼠肾细胞凋亡可能与氟所导致的DNA损伤有关。
[Abstract]:Objective to study the effects of fluoride on DNA damage and apoptosis in rat renal cells. Methods Forty-eight SD rats were randomly divided into 4 groups (control group, low fluorine group, middle fluorine group and high fluorine group), 12 rats in each group, drinking deionized water containing sodium fluoride for 0 ~ 50100 and 200 mg/L, respectively, and exposed to deionized water for 120 days. After the animals were killed, single cell gel electrophoresis (SCGE) was used to detect the DNA damage of rat renal cells induced by fluoride, and flow cytometry (FCM) was used to detect apoptosis. Results Comet assay showed that the tail rate of renal cells in each fluorine group was significantly higher than that in control group (P 0.01). The length of DNA migration of renal cells in fluoride group was higher than that in control group, the difference between high fluoride group and control group was statistically significant (P 0.01), and there was significant difference between middle fluoride group and control group (P 0.05). The results of flow cytometry showed that the apoptosis rate of renal cells in low fluorine group was significantly higher than that in control group, and that in middle fluorine group and high fluoride group was significantly higher than that in control group. There was a positive correlation between DNA damage and apoptosis in rat renal cells. Conclusion fluoride can induce DNA damage and apoptosis of renal cells in rats. Fluoride induced apoptosis of renal cells may be related to DNA damage induced by fluoride.
【作者单位】：山西医科大学实验动物中心;
【基金】：山西省实验动物专项资金(2012K02)
【分类号】：R363

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