大鼠脑干连续冰冻切片在NOS阳性神经核团三维重建中的应用

发布时间：2018-06-07 23:53

本文选题：三维重建 + NOS阳性核团　；参考：《神经解剖学杂志》2013年02期

【摘要】：目的:探讨应用组织学方法对大鼠脑干内一氧化氮合酶(nitric oxide synthase,NOS)阳性神经核团进行三维重建的技术。方法:以4只成年雄性SD大鼠为材料,标本固定后取中脑至脊髓颈1节段组织。3只于冰冻切片机上行冠状面连续切片,所得切片行NADPH-黄递酶(nicotinamide aenine inucleotide phosphate-diaphorase)染色,每张切片厚度为30μm。1只行正中矢状切片。所得切片行LFB(Luxol fast blue)染色,5倍物镜下行显微照相。应用Photoshop7.0软件拼接图像,并以三轴法将图片配准;应用3D-DOCTOR 4.0软件对连续图像中NOS阳性核团进行三维可视化重建,参照大鼠脑立体定位图谱确定核团名称,并利用软件自带工具计算核团体积。结果:三维重建的NOS阳性神经核团分布于中脑背侧、中脑导水管周围、桥脑基底部外侧、延脑腹侧外部。脑干内体积最大的NOS阳性神经核团为被盖背外侧核(laterodorsal tegmentum,LDTg),平均体积为0.6747±0.0026mm3。结论:利用组织学方法可以对大鼠脑干内NOS阳性神经核团进行三维可视化重建;借助3D-DOCTOR 4.0软件可对重建核团进行测量并计算出其体积。
[Abstract]:Aim: to investigate the technique of 3D reconstruction of nitric oxide synthase (oxide) synthase positive nuclei in the brain stem of rats by histological method. Methods: four adult male Sprague-Dawley rats were used as materials. After fixation, 3 segments of cervical tissue from midbrain to spinal cord were taken from frozen microtome. The sections were stained with NADPH-diaphorase (NADPH-diaphorase) aenine inucleotide phosphate-diaphorase (NADPH-diaphorase). The thickness of each section was 30 渭 m. 1. Only the median sagittal section was performed. The sections were stained with LFBX Luxol fast blue. and 5 times of objective lens was taken. Photoshop 7.0 software was used to concatenate the images and the images were registered by triaxial method, and 3D-DOCTOR 4.0 software was used to reconstruct the NOS positive nuclei in the continuous images, and the names of the nuclei were determined by referring to the stereotaxic map of the rat brain. The volume of nuclei was calculated by using software tools. Results: the NOS positive nuclei were located in the dorsal midbrain, periaqueductal midbrain, lateral base of pontine base and ventral side of the medulla oblongata. The largest NOS-positive nucleus in the brain stem was lateral odorsal tegmentumn (LDT), with an average volume of 0.6747 卤0.0026mm ~ 3. Conclusion: the NOS positive neuronal nuclei in the brain stem of rats can be reconstructed visually by histological method, and the volume of the reconstructed nuclei can be measured by 3D-DOCTOR 4.0 software and the volume of the reconstructed nuclei can be calculated by 3D-DOCTOR 4.0 software.
【作者单位】：南通大学医学院人体解剖学系;贵州省贵阳医学院人体解剖学教研室;南通大学医学信息学院;
【基金】：南通大学自然科学基金项目(102049、09Z032) 江苏高校优势学科建设工程资助项目南通市应用研究计划课题(BK2011055)
【分类号】：R329

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