胆固醇致内皮细胞损伤对平滑肌细胞增殖和迁移的促进作用

发布时间：2018-06-09 00:18

本文选题：动脉粥样硬化 + 血管内皮细胞　；参考：《第三军医大学学报》2013年13期

【摘要】：目的观察胆固醇损伤人血管内皮细胞(human umbilical vein endothelial cell,HUVEC)后能否影响血管平滑肌(vascular smooth muscle,VSMC)的增殖与迁移。方法用12.5、25.0、50.0 mg/L 3个浓度的胆固醇作用体外培养的HUVEC 24 h后,再用培养内皮细胞的培养基继续培养VSMC 24 h,CCK-8、划痕实验检测VSMC增殖与迁移;用12.5、25.0、50.0 mg/L的胆固醇作用共培养的HUVEC、VSMC 24 h,用Transwell检测VSMC迁移。结果 25.0、50.0 mg/L胆固醇作用HUVEC后的培养基与对照组相比(1.37±0.01),明显促进VSMC增殖(1.53±0.06)、(1.54±0.10)(P0.05);12.5、25.0、50.0 mg/L胆固醇作用HUVEC后的培养基与对照组相比(33.24±0.43)均能促进VSMC细胞迁移[(269.10±3.78)、(272.79±4.69)、(458.69±4.78),P0.01]。胆固醇作用共培养的HUVEC、VSMC 24 h后,促进VSMC迁移[(175.00±4.63)、(182.00±4.13)、(207.00±5.59)],与对照组相比(101.00±2.33)差异明显(P0.01)。结论胆固醇诱导HUVEC损伤后能够促进VSMC增殖与迁移。
[Abstract]:Objective to investigate the effects of cholesterol on the proliferation and migration of vascular smooth muscle cells (smooth muscleus) after cholesterol injury of human vascular endothelial cells (HUVECs). Methods HUVEC cultured in vitro was treated with 12.5U 25.0 mg / L cholesterol for 24 h, then cultured on the culture medium of VSMC for 24 h. The proliferation and migration of VSMC were detected by scratch test. HUVECs VSMC were co-cultured with 12.5U 25.0 mg / L cholesterol for 24 h. The migration of VSMC was detected by Transwell. Results compared with the control group, the culture medium of HUVEC treated with 25.0% 50.0 mg / L cholesterol significantly promoted the proliferation of VSMC (1.53 卤0.06) and significantly promoted the proliferation of VSMC (1.54 卤0.10). The culture medium of HUVEC treated with 25.0 mg / L cholesterol was 33.24 卤0.43), which could promote the migration of VSMC cells [269.10 卤3.782.79 卤4.698.69 卤4.78P0.01]. The VSMC of HUVECs co-cultured with cholesterol for 24 h promoted the migration of VSMC [175.00 卤4.63.00 卤4.13C, 207.00 卤5.59], which was significantly different from that of the control group (101.00 卤2.33). Conclusion cholesterol induced injury of HUVEC can promote proliferation and migration of VSMC.
【作者单位】：贵州省遵义医学院生物化学教研室;
【基金】：贵州省科技厅自然科学基金[黔科合J字(2009)2-178]~~
【分类号】：R329

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