高原低氧对小鼠脾脏T淋巴细胞亚群及其功能的影响

发布时间：2018-06-09 23:08

本文选题：高原低氧 + T淋巴细胞　；参考：《青海大学》2017年硕士论文

【摘要】：目的:研究高原低氧条件下,小鼠脾脏T淋巴细胞亚群及其功能的改变。方法:分别在400m,2200m和4200m的不同海拔条件下暴露小鼠30d后,ELISA法检测脾细胞培养上清中IL-4、IFN-γ的浓度;MTT法分析小鼠脾脏T淋巴细胞增殖能力的变化;流式细胞术分析法观察小鼠脾脏T淋巴细胞亚群及其表面活化共刺激分子表达的变化。结果:高海拔低氧暴露30d后,海拔4200 m和2200 m实验组小鼠与对照组(海拔400 m)相比,脾脏指数明显增高(P0.05),同时,4200 m低氧组小鼠脾脏指数与2200 m低氧组相比也显著增加(P0.05);小鼠脾细胞培养上清中IFN-γ的浓度明显降低(P0.05),而IL-4的浓度未发生明显变化;脾脏T淋巴细胞增殖能力明显降低(P0.05),同时,4200 m低氧组与2200 m低氧组相比,其T细胞增殖能力也显著下降(P0.05);脾脏CD3+、CD4+、CD8+T淋巴细胞数显著下降(P0.05),其中,CD4+T细胞数较CD8+T细胞数下降水平高;CD4+CD28+T细胞数也明显减少(P0.05),而CD8+CD28+T细胞无显著变化,另外,4200 m低氧组与2200 m低氧组相比,CD3+、CD4+以及CD4+CD28+T细胞数也明显减少(P0.05)。结论:初步说明高海拔低氧条件暴露,可引起小鼠脾脏T淋巴细胞数目减少且增殖能力降低,CD4+T淋巴细胞的活化水平显著降低,其分泌细胞因子的能力也发生改变,从而导致T细胞介导的特异性细胞免疫功能受到抑制。
[Abstract]:Objective: to study the changes of T lymphocyte subsets and their functions in mice spleen under high altitude hypoxia. Methods: after the mice were exposed at different altitude of 400m ~ 2200m and 4200m for 30 days, the concentration of IL-4 ~ (-4) -IFN- 纬 in the supernatant of spleen cell culture was determined by Elisa. The proliferation ability of T lymphocytes in spleen of mice was determined by MTT method. The changes of spleen T lymphocyte subsets and their surface activated costimulatory molecules were observed by flow cytometry. Results: after exposure to hypoxia at high altitude for 30 days, the mice in the experimental group of 4200 m and 2200 m were compared with the control group (400m above sea level). The spleen index of the mice in the 4200m hypoxia group was significantly higher than that in the 2200 m hypoxia group, and the concentration of IFN- 纬 in the supernatant of spleen cell culture decreased significantly, but the concentration of IL-4 did not change significantly. The proliferation ability of splenic T lymphocytes was significantly decreased compared with that of 2200 m hypoxia group. The proliferation ability of T cells was also significantly decreased (P 0.05) and the number of CD 4 / CD 8 T lymphocytes in spleen decreased significantly (P 0 05). The number of CD 4 T cells in the spleen was significantly lower than that in CD 8 T cells, and the number of CD 4 CD 28 T cells was significantly lower than that of CD 8 T cells, while the number of CD 8 CD 28 T cells had no significant change. In addition, the number of CD4 and CD4 CD28T cells in 4200m hypoxia group was significantly lower than that in 2200 m hypoxia group. Conclusion: exposure to hypoxia at high altitude can decrease the number of T lymphocytes in spleen, decrease the activity of CD4 T lymphocytes and change the ability of secreting cytokines. As a result, T cell mediated specific cellular immune function was inhibited.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R392

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