干扰素诱导蛋白16对人脑血管外膜成纤维细胞增殖与迁移的影响及其机制

发布时间：2018-06-14 22:42

本文选题：干扰素诱导蛋白 + 血管外膜成纤维细胞　；参考：《中国病理生理杂志》2013年04期

【摘要】：目的:研究干扰素诱导蛋白16(IFI16)对人脑血管外膜成纤维细胞(HBVAFs)增殖与迁移的影响及其可能机制。方法:在HBVAFs中转染针对IFI16基因的小分子干扰RNA(siRNA)48 h后,用2×106U/L干扰素-α(IFN-α)处理转染IFI16 siRNA细胞24 h。流式细胞术测定细胞周期,细胞划线法与Transwell法测定细胞迁移能力。应用real-time PCR法和蛋白免疫印迹(Western blotting)法分别测定细胞中IFI16、p53及p21 mRNA和蛋白表达水平的变化。结果:转染IFI16 siRNA后,HBVAFs中IFI16、p53及p21 mRNA和蛋白表达水平下调,增加了细胞G1/S期转换。IFN-α可诱导HBVAFs中IFI16、p53及p21mRNA和蛋白表达水平上调,同时抑制细胞G1/S期转换与细胞迁移,但在转染了IFI16 siRNA的HBVAFs中IFN-α的上述作用受到抑制。结论:IFI16表达可以抑制HBVAFs增殖和迁移,其机制可能与激活p53和p21的表达有关。
[Abstract]:Aim: to investigate the effects of interferon inducible protein 16 (IFI16) on the proliferation and migration of human cerebrovascular adventitia fibroblasts (HBVAFs) and its possible mechanism. Methods: small interfering RNAs targeting IFI16 gene were transfected into HBV AFs for 48 h. IFI16 siRNA cells were transfected with IFI16 siRNA cells for 24 h with 2 脳 106 UUL interferon- 伪 (IFN- 伪). Cell cycle was measured by flow cytometry, cell migration was measured by cell line method and Transwell method. The expression of p53 and p21 mRNA and protein were detected by real-time polymerase chain reaction and Western blotting. Results: after transfection of IFI16 siRNA, the expression levels of p53 and p21 mRNA and protein in HBV AFs were down-regulated, which increased the expression level of IFI16 p53 and p21 mRNA and protein in HBV AFs, and inhibited the G1 / S phase transition and cell migration. But IFN- 伪 was inhibited in HBV AFs transfected with IFI16 siRNA. Conclusion the expression of 1: IFI16 can inhibit the proliferation and migration of HBV AFs, and its mechanism may be related to the activation of p53 and p21 expression.
【作者单位】：贵州省人民医院心内科;
【基金】：国家自然科学基金资助项目(No.81260030) 贵州省高层次人才科研条件特助经费项目合同(No.TZJF-2010年-098号) 贵州省科技攻关项目(黔科合LS字[2012]021号)
【分类号】：R363

【参考文献】