小鼠急性脂肪栓塞综合征模型中炎症因子及炎症信号通路的激活

发布时间：2018-06-17 10:12

本文选题：脂肪栓塞综合征 + 半数致死剂量　；参考：《上海交通大学学报(医学版)》2013年02期

【摘要】：目的研究急性脂肪栓塞动物模型中炎症因子的激活及可能的信号转导途径。方法经健康雄性C57小鼠尾静脉注射同种异体小鼠肾周脂肪建立小鼠脂肪栓塞模型,采用Bliss法求出脂肪栓塞的半数致死剂量(LD50)。比较实验组(静脉注射LD50的脂肪)和对照组(给予相同剂量的生理盐水)大体和镜下肺组织表现、动脉血气、肺组织干湿质量比值(D/W)、肺组织髓过氧化物酶(MPO)活性、血浆IL-1β、TNF-α水平以及肺组织匀浆液中IKKβ基因和NF-κB蛋白的表达。结果与对照组相比,实验组肺组织呈严重损伤表现,血管腔和肺泡腔中有脂滴存积,肺组织D/W明显上升;实验组小鼠动脉血PaO2明显降低,PaCO2升高,血浆IL-1β和TNF-α水平显著上升,肺组织MPO活性升高,IKKβ基因表达上调,核转录因子NF-κB表达升高(P0.05)。结论炎症反应在脂肪栓塞综合征病理过程中发挥了重要作用,NF-κB相关基因和蛋白表达升高提示NF-κB介导的炎症信号通路参与了脂肪栓塞激发的炎症病理过程。
[Abstract]:Objective to study the activation of inflammatory factors and the possible signal transduction pathway in acute fat embolism animal model. Methods the fat embolization model of mice was established by injecting allogenic perirenal fat into tail vein of healthy male C57 mice. The LD50 dose of fat embolism was calculated by Bliss method. The changes of lung tissue, arterial blood gas, lung tissue dry-wet mass ratio (D- WN) and myeloperoxidase (MPO) activity were compared between the experimental group (LD50 fat) and the control group (given the same dose of normal saline) under microscope, compared with that of the experimental group (intravenous injection of LD50 fat) and the control group (given the same dose of normal saline). The expression of IKK 尾 gene and NF- 魏 B protein in lung tissue homogenate. Results compared with the control group, the lung tissue in the experimental group showed severe injury, lipid accumulation in the blood lumen and alveolar cavity, and D- / W in the lung tissue increased significantly, PaO2 in the arterial blood of the experimental group decreased significantly, and the plasma levels of IL-1 尾 and TNF- 伪 increased significantly in the experimental group compared with the control group, and the levels of plasma IL-1 尾 and TNF- 伪 increased significantly in the experimental group. The expression of IKK 尾 gene was up-regulated and the expression of nuclear transcription factor NF- 魏 B was increased in lung tissue. Conclusion inflammatory reaction plays an important role in the pathogenesis of fat embolism syndrome. The increased expression of NF- 魏 B related genes and proteins suggests that NF- 魏 B mediated inflammatory signaling pathway is involved in the inflammatory pathological process induced by fat embolism.
【作者单位】：上海交通大学附属第六人民医院麻醉科;焦作市妇幼保健院儿外科;
【基金】：国家自然科学基金(81071591)~~
【分类号】：R-332

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