神经调节蛋白-1β在压力超负荷大鼠肥大心肌组织中的表达下降

发布时间：2018-06-19 03:27

本文选题：NRG-β + 压力超负荷　；参考：《基础医学与临床》2013年11期

【摘要】：目的研究NRG-1β在压力超负荷心肌肥大大鼠心肌中的变化。方法复制心肌肥大模型,将模型及假手术动物各随机分成2组:4周模型组(4wM)和8周模型组(8wM组)。采用心动超声及血流动力学评价心功能;Masson染色观察心肌结构;放射免疫法检测心肌中血管紧张素Ⅱ(AngⅡ)含量;Real-time PCR及Westrrn blot检测心肌中神经调节蛋白-1(NRG-1β)mRNA及蛋白的表达。结果 1)和4周对照组(4wC组)比较,4wM组左室射血分数(LVEF)、短轴缩短率(LVFS)、左室收缩末压(LVESP)、左室内压最大上升和下降速率(±dP/dt max)下降(P0.05),左室收缩末内径(LVEDD)、舒张末内径(LVESD)、左室舒张末压(LVEDP)及心肌胶原容积分数(CVF)增高(P0.05,P0.01),心肌中AngⅡ表达增加(P0.01)。2)和8周模型组(8wC组)比较,8wM组LVEF、LVFS、LVESP、±dP/dt max下降(P0.01),LVEDD、LVESD、LVEDP及CVF增高(P0.05,P0.01),心肌中AngⅡ表达增加,NRG-1βmRNA及蛋白表达下降(P0.01)。结论 NRG-1β在压力超负荷心肌肥大大鼠心肌中表达下调,可能参与了压力超负荷所致心肌肥大的发生和发展。
[Abstract]:Objective to study the changes of NRG-1 beta in myocardium of hypertrophic myocardial hypertrophy in rats. Methods the model of myocardial hypertrophy was replicated and the models and sham operated animals were randomly divided into 2 groups: 4 weeks model group (4wM) and 8 week model group (group 8wM). Cardiac function was evaluated by echocardiography and hemodynamics; Masson staining was used to observe the myocardial structure; radioimmunoassay was used to detect the myocardium. The content of angiotensin II (Ang II) in the myocardium; Real-time PCR and Westrrn blot to detect the expression of -1 (NRG-1 beta) mRNA and protein in the myocardium. Results 1) compared with the 4 week control group (4wC group), the left ventricular ejection fraction (LVEF), the short axis shortening rate (LVFS), the left ventricular systolic pressure, the maximum increase and decline rate of left indoor pressure in the control group (4wC group) were compared with the control group (4wC group). P/dt max) decreased (P0.05), left ventricular end systolic diameter (LVEDD), terminal diastolic diameter (LVESD), left ventricular end diastolic pressure (LVEDP) and myocardial collagen volume fraction (CVF) increased (P0.05, P0.01), Ang II in myocardium (P0.01).2) and 8 weeks model group. .05, P0.01), the expression of Ang II in myocardium increased and NRG-1 beta mRNA and protein expression decreased (P0.01). Conclusion NRG-1 beta was downregulated in myocardium of hypertrophic myocardium in rats with pressure overload, which may be involved in the occurrence and development of myocardial hypertrophy induced by pressure overload.
【作者单位】：延安大学医学院护理系;延安市卫生学校护理中心;延安大学附属医院病理科;
【分类号】：R363

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