NDGA对移植入损伤脊髓后的化学去细胞肌肉支架中的羊膜上皮细胞存活的影响

发布时间：2018-07-07 14:06

本文选题：NDGA + 化学去细胞肌肉支架　；参考：《吉林大学学报(医学版)》2013年06期

【摘要】：目的:观察NDGA对植入大鼠脊髓损伤处的化学去细胞肌肉支架中的羊膜上皮细胞(AECs)存活的影响,为NDGA在脊髓损伤中的进一步应用提供实验依据。方法:制备化学去细胞肌肉支架并联合Dil标记的6只孕鼠AECs用于大鼠脊髓半横断损伤。将72只模型鼠随机分成生理盐水组(n=18)、20mg·kg-1 NDGA组(n=18)、30mg·kg-1 NDGA组(n=18)和40mg·kg-1 NDGA组(n=18),各组大鼠分别于术后1周每天注射生理盐水和20、30、40mg·kg-1 NDGA,并分别于术后1、2和4周取材,荧光显微镜下观察并比较各组大鼠AECs存活的数量;采用免疫组织化学方法观察各组大鼠损伤脊髓新生血管阳性面积百分比和NG2阳性内源性神经干细胞的数量。结果:各组大鼠支架中AECs的数量随着时间的延长均不断减少,移植后1、2和4周各时间点,30mg·kg-1 NDGA组和40mg·kg-1 NDGA组大鼠支架中AECs的存活数量明显高于生理盐水组和20mg·kg-1 NDGA组(P0.05);移植1周后,30mg·kg-1 NDGA组和40mg·kg-1 NDGA组大鼠新生血管阳性面积百分比明显低于生理盐水组和20mg·kg-1 NDGA组(P0.05);移植1周后,各组大鼠间NG2阳性细胞数量差异无统计学意义(P0.05)。结论:NDGA能够明显增加大鼠脊髓损伤后的化学去细胞肌肉支架中移植AECs的存活数量,对损伤脊髓新生血管具有抑制作用,而对于内源性神经干细胞无影响。
[Abstract]:Aim: to observe the effect of NDGA on the survival of amniotic epithelial cells (AECs) implanted into the chemically acellular muscle scaffold of spinal cord injury in rats, and to provide experimental evidence for the further application of NDGA in spinal cord injury. Methods: chemically acellular muscle scaffolds were prepared and combined with Dil labeled AECs in 6 pregnant rats for spinal cord hemitransection injury. Seventy-two model rats were randomly divided into two groups: normal saline group (NN18), 20mg kg-1 NDGA group (NN18), 30mg kg-1 NDGA group (nni18) and 40mg kg-1 NDGA group (NN18). The rats in each group were injected with normal saline and 200.30U 40mg kg-1 NDGA1 week after operation, and the samples were obtained at 12 and 4 weeks postoperatively. The number of AECs survival was observed under fluorescence microscope and the percentage of neovascularization positive area and the number of NG2 positive neural stem cells were observed by immunohistochemical method. Results: the number of AECs in the stents decreased with time. The survival amount of AECs in the stents of 30mg kg-1 NDGA group and 30 mg 40mg kg-1 NDGA group was significantly higher than that of normal saline group and 20mg kg-1 NDGA group at 1 and 4 weeks after transplantation (P0.05), and the percentage of neovascularization positive area in 30 mg kg-1 NDGA group and 40mg kg-1 NDGA group was significantly higher than that in normal saline group and 20mg kg-1 NDGA group 1 week after transplantation (P0.05). It was lower than normal saline group and 20mg kg-1 NDGA group (P0.05), 1 week after transplantation, There was no significant difference in the number of NG2 positive cells among groups (P0.05). Conclusion WNDGA can significantly increase the survival rate of AECs in the chemically acellular muscle scaffolds after spinal cord injury in rats, and inhibit the neovascularization of injured spinal cord, but have no effect on endogenous neural stem cells.
【作者单位】：吉林大学护理学院基础护理教研室;吉林大学白求恩医学院组织学与胚胎学系;吉林大学中日联谊医院骨科;吉林省妇幼保健院眼科;广东医学院组织学与胚胎学教研室;
【基金】：国家自然科学基金资助课题(30970739)
【分类号】：R329

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