抑郁型动脉粥样硬化大鼠模型的建立与评价

发布时间：2018-07-07 17:40

本文选题：抑郁 + 动脉粥样硬化　；参考：《中国药理学通报》2013年07期

【摘要】：目的探讨建立抑郁型动脉粥样硬化大鼠模型的实验方法并评价模型的有效性。方法给大鼠喂食高脂饲料,利用慢性不可预见性应激造成抑郁表现,观察动物摄食量的变化,用旷场试验测定动物行为,检测血清学生化指标,HE染色观察腹主动脉和肝组织病变情况。结果与正常组相比,模型组大鼠食物消耗量明显减少(P0.01);旷场实验中垂直得分、水平得分、总得分均明显降低(P0.01);肝、脾、胸腺的脏器系数明显减少(P0.01);血清中总胆固醇(TC)、低密度脂蛋白(LDL)、白蛋白(ALB)、球蛋白(GLB)、碱性磷酸酶(ALP)、丙二醛(MDA)含量明显升高(P0.01),高密度脂蛋白(HDL)含量明显降低(P0.01),超氧化物歧化酶(SOD)活性降低(P0.05);主动脉内膜-中膜明显增厚、内膜单核细胞、巨噬细胞浸润与聚集增多,内膜表层可见脂纹脂斑病变。结论高脂饲料饲养叠加慢性不可预见性应激,可造成大鼠兼具动脉粥样硬化和抑郁两方面的症状,与伴有抑郁症的动脉粥样硬化病人的临床表现有较好的相似性,是较理想的动物模型。
[Abstract]:Objective to explore the experimental method and evaluate the effectiveness of depressive atherosclerosis rat model. Methods Rats were fed with high fat diet, and depression was induced by chronic unpredictable stress. The changes of food intake were observed, and the behavior of animals was measured by open field test. The pathological changes of abdominal aorta and liver were observed by HE staining. Results compared with the normal group, the food consumption in the model group was significantly decreased (P0.01), the vertical score, horizontal score and total score were significantly decreased in the open field experiment (P0.01); The organ coefficient of thymus significantly decreased (P0.01), serum total cholesterol (TC), low density lipoprotein (LDL), albumin (ALB), globulin (GLB), alkaline phosphatase (ALP), malondialdehyde (MDA) content increased significantly (P0.01), high density lipoprotein (HDL) content decreased significantly (P0.01), superoxide content (P0.01) The activity of SOD was decreased (P0.05), the intima-media of aorta was obviously thickened, The infiltration and aggregation of monocytes and macrophages in the intima were increased, and lipid plaques were found in the surface layer of the intima. Conclusion the superposition of chronic unpredictable stress with high fat diet can cause both symptoms of atherosclerosis and depression in rats, and there is a good similarity with the clinical manifestations of atherosclerotic patients with depression. It is an ideal animal model.
【作者单位】：广州中医药大学中药学院;
【基金】：国家自然科学基金资助项目(No 81102530) 广东省代谢性疾病中医药防治重点实验室项目
【分类号】：R-332

【参考文献】