云南蝙蝠轮状病毒的分离与鉴定

发布时间：2018-07-13 10:26

【摘要】：蝙蝠是携带人兽共患病毒最多的一种哺乳动物,调查蝙蝠携带病毒的病原生态学本底对防范蝙蝠病毒威胁人类健康具有重要意义。本研究采集云南省部分地区蝙蝠进行病毒宏基因组学分析,在一组食虫蝙蝠样品中发现了A群轮状病毒(Group A rotaviruses,RVA)序列,经过进一步RT-PCR筛查验证及病毒的分离鉴定,最终从勐远县的1只三叶蹄蝠中分离出一株轮状病毒。扩增该毒株的VP7与VP4基因进行分型与系统进化分析,结果表明其VP7基因为G3型,与来自阿根廷的1株马轮状病毒的同源性最高,为93%;其VP4基因为P[10]型,与来自泰国的1株人轮状病毒同源性最高,为94.8%。通过与本实验室之前分离的首株蝙蝠G3P[3]型轮状病毒MSLH14的比较,确定该毒株为一株新的蝙蝠轮状病毒,命名为RVA/Bat-tc/MYAS33/2013/G3P[10],简称MYAS33。本研究结果进一步证明了蝙蝠轮状病毒的多样性,突显了蝙蝠作为轮状病毒潜在宿主的重要性。
[Abstract]:Bat is one of the most common mammals carrying zoonotic virus. It is very important to investigate the ecological background of bat carrying virus to prevent bat virus from threatening human health. Group A rotavirus (Group A) rotavirus (RVA) sequence was found in a group of insectivorous bat samples from some bats in Yunnan Province, and was further screened by RT-PCR and identified by virus isolation. Finally, a rotavirus was isolated from a three-leaf bat in Mengyuan County. The results of genotyping and phylogenetic analysis of VP7 and VP4 showed that the VP7 gene had the highest homology with that of a horse-rotavirus strain from Argentina, and its VP4 gene was of P [10] type. It had the highest homology with a strain of human rotavirus from Thailand (94.8%). Compared with the first rotavirus MSLH14 isolated previously in our laboratory, the strain was identified as a new bat rotavirus named RVA / Bat-tcr / MYAS33 / G3P [10]. The results further demonstrated the diversity of rotavirus in bats and highlighted the importance of bats as potential hosts of rotavirus.
【作者单位】：吉林农业大学动物科技学院;军事医学科学院军事兽医研究所吉林省人兽共患病防控重点实验室;成都军区疾病预防控制中心;云南省动物疫病预防控制中心;
【基金】：NSFC云南省联合基金(U1036601) “973”计划(2012AA022006)资助
【分类号】：R373

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