精神应激致内脏高敏感大鼠的心理行为学变化

发布时间：2018-07-16 20:13

【摘要】：目的观察应用精神应激方法复制内脏高敏感动物模型过程中大鼠的心理行为学变化。方法采用持续避水应激刺激方法复制内脏高敏感动物模型,通过分析结直肠扩张(colorectal distention,CRD)诱发的腹壁收缩反射(AWR)及肌电活动改变对模型进行鉴定,在制模过程中持续观察大鼠体质量、肠道运动(排便颗粒)改变并采用旷场实验观察大鼠的心理行为学变化。结果①应激组的大鼠60 mmHg CRD压力刺激下的AWR评分及VMR与假性应激组和空白对照组相比,差异均有统计学意义;②随着应激天数的增加,应激组的大鼠的体质量逐渐下降,应激前后比较差异有统计学意义(P0.05),且随着应激天数的增加,排粪粒数逐渐下降,但应激前后比较差异无统计学意义(P=0.172);③应激刺激后大鼠的站立次数及跨越分格线数显著下降,差异有统计学意义(P0.05)。结论持续避水应激刺激可成功复制内脏高敏感动物模型,显著抑制大鼠体质量增加,改变其肠道动力,降低大鼠兴奋性,诱发其抑郁样行为,为其潜在机制的进一步研究提供实验基础。
[Abstract]:Objective to observe the changes of psychological behavior in rat model of visceral hypersensitivity induced by mental stress. Methods the visceral hypersensitive animal model was induced by continuous water avoidance stress. The model was identified by analyzing the abdominal wall contraction reflex (awr) and the changes of myoelectric activity induced by colorectal distentification. The changes of body mass, bowel movement (defecation granules) and psychological behavior of rats were observed in the course of modeling. Results 1 the AWR score and VMR of the stress group under 60 mmHg CRD stress were significantly different from those of the sham stress group and the blank control group. With the increase of the stress days, the body weight of the stress group decreased gradually. The difference before and after stress was statistically significant (P0.05), and with the increase of stress days, the number of excrement grains decreased gradually, but there was no significant difference before and after stress (P0. 172). The difference was statistically significant (P0.05). Conclusion continuous water-avoidance stress stimulation can successfully replicate the visceral hypersensitive animal model, significantly inhibit the increase of body mass, change the intestinal motility, decrease the excitability and induce depressive behavior in rats. It provides experimental basis for further study of its potential mechanism.
【作者单位】：同济大学附属同济医院消化内科;
【基金】：国家自然科学基金(No:81370491) 上海市卫生局青年科研项目(No:20114Y085)
【分类号】：R-332

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