壬基酚对雌性SD大鼠胸腺及淋巴细胞损伤作用

发布时间：2018-08-07 07:48

【摘要】：目的探讨环境雌激素壬基酚(NP)暴露对雌性SD大鼠胸腺组织及胸腺淋巴细胞的损伤作用。方法将32只健康8～9周龄SPF级雌性SD大鼠按体重随机分为溶剂对照组(玉米油)和低(20 mg/kg)、中(80 mg/kg)、高(200 mg/kg)剂量NP组,每组8只,采用灌胃方式染毒,灌胃容量5 ml/kg,隔天染毒1次,连续60 d,观察胸腺组织病理学改变;采用流式细胞仪检测胸腺淋巴细胞内活性氧(ROS)、游离钙离子([Ca2+]i)浓度、线粒体膜电位(MMP)水平变化。结果溶剂对照组大鼠胸腺重量为(0.633±0.092)g,高剂量NP组为(0.514±0.084)g,差异有统计学意义(P0.05),胸腺重量和染毒剂量呈负相关(r=-0.317,P0.05);溶剂对照组与高剂量NP组大鼠胸腺淋巴细胞内ROS水平分别为(4.59±0.25)和(5.57±0.36),差异有统计学意义(P0.05),胸腺淋巴细胞内ROS水平与NP浓度呈正相关(r=0.486,P0.05);病理学检查可见,中、高剂量NP组胸腺皮质变薄。结论 NP对雌性大鼠胸腺及胸腺淋巴细胞具有一定毒性作用,并可引起免疫功能损伤。
[Abstract]:Objective to investigate the effects of environmental estrogen nonylphenol (NP) exposure on thymus tissue and thymic lymphocytes in female SD rats. Methods 32 healthy SPF female SD rats of 9 weeks old were randomly divided into solvent control group (corn oil) and low (20 mg/kg), middle (80 mg/kg), high (200 mg/kg) dose NP groups (n = 8 in each group). The histopathological changes of thymus were observed by intragastric perfusion of 5 ml / kg once every other day for 60 days, the concentration of free calcium ([Ca2] I) of reactive oxygen species ([Ca2] I) in thymic lymphocytes and the (MMP) level of mitochondrial membrane potential were detected by flow cytometry. Results the weight of thymus was (0.633 卤0.092) g in solvent control group and (0.514 卤0.084) g in high dose NP group, the difference was statistically significant (P0.05). There was a negative correlation between thymus weight and exposure dose (r-0.317g), and the ROS level in thymus lymphocytes in solvent control group and high dose NP group was respectively. (4.59 卤0.25) and (5.57 卤0.36), the difference was statistically significant (P0.05). The level of ROS in thymus lymphocytes was positively correlated with the concentration of NP (r = 0.486P 0.05). Thymic cortex thinned in middle and high dose NP groups. Conclusion NP has some toxic effects on thymus and thymus lymphocytes of female rats, and can cause damage to immune function.
【作者单位】：苏州大学医学部公共卫生学院劳动卫生与环境卫生教研室;江苏省放射医学与防护重点实验室;
【基金】：教育部留学回国人员基金(K5126504)
【分类号】：R363

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