埃及伊蚊性别决定基因Transformer 2的鉴定与表达分析

发布时间：2018-08-20 17:51

【摘要】：目的分离、鉴定和分析埃及伊蚊性别决定基因Transformer 2(Aaetra2)。方法通过生物信息学方法与分子生物学方法获得了Aaetra2基因的全长。通过与已知物种tra2的比较,分析Aaetra2的基因结构特点与分子进化特征,通过RT-PCR方法验证Aaetra2的时间与组织表达特点。结果分离并鉴定Aaetra2-α与Aaetra2-β两个基因,它们位于染色体不同部位,通过选择性启动子的剪切策略进行自我调控,转录多种mRNA。埃及伊蚊TRA2蛋白具有保守的RRM、RS1、RS2和linker功能结构域区。Aaetra2表达方式无时间特异性的,从卵持续性表达至成虫阶段,且无性别特异性表达与组织特异性表达。结论 Aaetra2具有保守的昆虫性别决定基因tra2的功能结构域与表达特征,对其深入研究将为其最终应结合释放携带显性致死基因的昆虫技术应用于蚊虫的防制提供理论依据。
[Abstract]:Objective to isolate, identify and analyze the sex determinant gene Transformer 2 (Aaetra2) of Aedes aegypti. Methods the full length of Aaetra2 gene was obtained by bioinformatics and molecular biology. Compared with the known species tra2, the gene structure and molecular evolution characteristics of Aaetra2 were analyzed, and the time and tissue expression characteristics of Aaetra2 were verified by RT-PCR method. Results two genes, Aaetra2- 伪 and Aaetra2- 尾, were isolated and identified. They were located in different parts of chromosome. They were self-regulated by selective promoter and transcribed a variety of mRNAs. The expression of TRA2 protein in Aedes aegypti showed no time specificity in the conserved functional domain of RRMN RS1, RS2 and linker. It was expressed continuously from egg to adult, and there was no sex specific expression or tissue specific expression in Aedes aegypti. Conclusion Aaetra2 has the functional domain and expression characteristics of tra2, which is a conserved insect sex determinant gene, and its further study will provide a theoretical basis for the application of insect technology with dominant lethal gene to mosquito control.
【作者单位】：南方医科大学公共卫生与热带医学学院病原生物学系广东普通高校新发传染病防治重点实验室;
【基金】：国家自然科学基金(81271879)~~
【分类号】：R384

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