KIF1B在瘦素促进妊娠早期绒毛滋养细胞MMP-2表达中的作用

发布时间：2018-08-27 09:10

【摘要】：目的观察瘦素对妊娠早期绒毛滋养细胞基质金属蛋白酶2(MMP-2)表达的影响,探讨驱动蛋白家族成员1B(KIF1B)在瘦素对MMP-2调控中的作用。方法正常妊娠妇女人工流产绒毛组织(6～9周),按常规分离获得滋养细胞,分为对照组、leptin(100和500μg/L)刺激组以及KIF1B-siRNA、leptin(100和500μg/L)分别+KIF1B-siR-NA组,24 h后,明胶酶谱检测上清MMP-2的表达,RT-PCR检测MMP-2 mRNA、瘦素长型受体(OB-Rl)mRNA及KIF1B mRNA表达,Western blot检测KIF1B蛋白表达。结果与对照组相比,瘦素(100和500μg/L)显著促进滋养细胞MMP-2表达(100μg/L:mRNA水平由0.11±0.02增至0.18±0.05,P0.05);瘦素以剂量依赖方式促进OB-Rl及KIF1B表达(P0.05);KIF1B-siRNA部分抑制瘦素对MMP-2分泌的促进作用。结论瘦素可能通过lep-tin R-KIF1B途径促进MMP-2分泌,从而促进妊娠早期滋养细胞侵袭,为阐明滋养细胞侵袭调控机制提供了新的基础数据。
[Abstract]:Objective to investigate the effect of leptin on the expression of matrix metalloproteinase 2 (MMP-2) in trophoblast cells in early pregnancy and to explore the role of KIF1B in the regulation of MMP-2 by leptin. Methods Human chorionic villi of normal pregnant women (6 weeks) were divided into control group (100 渭 g / L and 500 渭 g / L) and KIF1B-siRNA,leptin (100 渭 g / L and 500 渭 g / L) for 24 h. The expression of MMP-2 in supernatant was detected by gelatin zymogram and the expression of MMP-2 mRNA, leptin long receptor (OB-Rl) mRNA and KIF1B mRNA were detected by RT-PCR. Western blot was used to detect the expression of KIF1B protein. Results compared with the control group, leptin (100 渭 g / L and 500 渭 g / L) significantly increased the expression of MMP-2 (from 0.11 卤0.02 to 0.18 卤0.05) in trophoblast, and leptin partially inhibited the expression of OB-Rl and KIF1B in a dose-dependent manner (P0.05). Conclusion leptin may promote the secretion of MMP-2 through lep-tin R-KIF1B pathway and thus promote the invasion of trophoblastic cells in early pregnancy. It provides new basic data for elucidating the mechanism of trophoblastic invasion regulation.
【作者单位】：山东大学齐鲁医院临床基础研究所;山东大学齐鲁医院检验科;山东大学齐鲁医院妇产科;
【基金】：国家自然科学基金(81072406,30872321) 山东省自然科学基金(Y2008C02) 山东大学研究生自主创新基金(yzc10133) 山东大学优秀研究生科研创新基金(yyx10126)
【分类号】：R329

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