二苯乙烯苷对同型半胱氨酸诱导血管内皮细胞凋亡及bcl-2、bax、caspase-3表达的影响

发布时间：2018-09-13 07:43

【摘要】：目的:研究何首乌二苯乙烯苷(TSG)对同型半胱氨酸(Hcy)诱导人脐静脉内皮细胞(HUVECs)凋亡及bcl-2、bax和caspase-3 mRNA表达的影响。方法:建立Hcy(3 mmol/L)所致培养HUVECs核损伤模型,TSG(1和10μmol/L)提前2 h预孵育,然后再以Hcy处理,作为TSG保护组。用Hoechst 33342核染色法观察HUVECs细胞核损伤状态,以流式细胞术检测细胞凋亡情况,用实时荧光定量RT-PCR法检bcl-2、bax和caspase-3 mRNA的表达。结果:经3 mmol/L Hcy处理后,与正常培养的细胞相比,HUVECs核损伤加重,凋亡细胞比例升高,bcl-2表达降低(P0.01),bax和caspase-3表达增加(P0.01)。TSG 1μmol/L和10μmol/L预孵育后再经3 mmol/L Hcy处理,与单经3 mmol/L Hcy损伤模型组相比,HUVECs细胞核损伤降低,凋亡率下降,bcl-2的表达增加(P0.05),bax和caspase-3表达降低(P0.05)。结论:TSG具有降低Hcy所致HUVECs的细胞损伤和抑制凋亡的作用,其机制可能与影响bcl-2、bax和caspase-3的表达有关。
[Abstract]:Aim: to investigate the effects of stilbene polygonum multiflorum (TSG) on (HUVECs) apoptosis and the expression of bcl-2,bax and caspase-3 mRNA in human umbilical vein endothelial cells induced by homocysteine (Hcy). Methods: Hcy (3 mmol/L) induced HUVECs nuclear injury model (1 and 10 渭 mol/L) was preincubated 2 hours in advance and then treated with Hcy as TSG protection group. The nuclear damage of HUVECs was observed by Hoechst 33342 nuclear staining, apoptosis was detected by flow cytometry, and the expression of bcl-2,bax and caspase-3 mRNA was detected by real-time fluorescence quantitative RT-PCR. Results: after 3 mmol/L Hcy treatment, the nuclear damage of HUVECs was more serious than that of normal cultured cells. The percentage of apoptotic cells increased and the expression of bcl-2 and caspase-3 increased (P0.01). TSG1 渭 mol/L and 10 渭 mol/L were preincubated with 3 mmol/L Hcy. Compared with the model group, the nuclear damage and apoptosis rate of HUVECs were decreased and the expression of bcl-2 was increased (P0.05) and the expression of caspase-3 was decreased (P0.05). Conclusion: TSG can reduce the damage of HUVECs cells induced by Hcy and inhibit apoptosis, and its mechanism may be related to the expression of bcl-2,bax and caspase-3.
【作者单位】：山东省医学科学院药物研究所;山东省医学科学院山东省罕少见病重点实验室;
【基金】：山东省自然科学基金资助项目(No.ZR2008C317)
【分类号】：R363

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