发酵、梨支原体感染小鼠小肠黏膜和胰腺组织细胞电镜观察

发布时间：2018-09-14 13:14

【摘要】：目的探讨发酵支原体(Mycoplasma fermentans,Mf)和梨支原体(Mycoplasma pirum,Mpi)感染致死小鼠小肠黏膜和胰腺组织细胞超微结构病理改变。方法 35只清洁级ICR小鼠,隔日腹腔注射环磷酰胺(50mg/kg.d)5次制成免疫抑制模型。分为Mf、Mpi感染组(n=12)和生理盐水(NS)对照组(n=11),分别腹腔注射0.3mL标准菌株Mf、Mpi(6×108-9/mL)和无菌生理盐水,计算各组死亡率,取小鼠血液进行支原体再培养以及感染12h、6d后取小肠黏膜和胰腺组织超微结构观察。结果免疫抑制小鼠Mf或Mpi感染6d后,全部死亡,支原体再培养阳性。电镜观察显示:Mf或Mpi感染12h后,小肠黏膜和胰腺组织细胞即表现超微结构病理改变,感染6d后,小肠黏膜组织细胞显著水肿,肿胀,线粒体空泡,胰腺细胞线粒体空泡,酶原颗粒变小,细胞变性、坏死,与NS组比较差异显著,且胰腺组织细胞病理改变比小肠黏膜明显。结论发酵支原体或梨支原体感染小鼠12h后,小肠黏膜和胰腺组织细胞即表现超微结构病理改变,6d后出现细胞水肿、变性及坏死。且胰腺组织细胞病理改变比小肠黏膜组织细胞更明显。
[Abstract]:Objective to investigate the ultrastructural changes of intestinal mucosa and pancreatic tissue cells in mice killed by mycoplasma fermentum (Mycoplasma fermentans,Mf) and mycoplasma pear (Mycoplasma pirum,Mpi). Methods 35 clean ICR mice were injected cyclophosphamide (50mg/kg.d) 5 times every other day to make immunosuppressive model. Mf,Mpi infection group (nong12) and saline (NS) control group (nong11) were injected intraperitoneally with standard 0.3mL strain Mf,Mpi (6 脳 108-9/mL) and aseptic saline, respectively. The mortality of each group was calculated. The blood of the mice was recultured and the ultrastructure of small intestine mucosa and pancreas were observed after infection for 12 h or 6 d. Results after 6 days of Mf or Mpi infection in immunosuppressive mice, all of them died and mycoplasma was positive again. Electron microscopic observation showed that intestinal mucosal and pancreatic tissue cells showed ultrastructural pathological changes 12 h after infection. After 6 days of infection, intestinal mucosal cells showed edema, swelling, mitochondrial vacuoles, mitochondria vacuoles and mitochondria vacuoles in pancreatic cells. Compared with NS group, the changes of proenzyme granules were smaller, cell denaturation and necrosis were significantly different, and the pathological changes of pancreatic tissue were more obvious than that of small intestinal mucosa. Conclusion after 12 hours of mycoplasma fermentum or mycoplasma pear infection, the cells of small intestine mucosa and pancreas showed ultrastructural pathological changes. After 6 days, the cells showed edema, degeneration and necrosis. The histopathological changes of pancreas were more obvious than those of small intestinal mucosa.
【作者单位】：温州医学院检验医学院和生命科学学院;
【基金】：温州市科技计划项目(Y20110107)~~
【分类号】：R375

【参考文献】