葡萄糖-6-磷酸脱氢酶缺乏对红细胞溶血率的影响

发布时间：2018-11-05 08:59

【摘要】：目的了解人红细胞葡萄糖-6-磷酸脱氢酶(G6PD)的活性与氧化性损伤的关系,及对红细胞溶血率的影响。方法选择医院门诊血标本共267例,采用WHO推荐的Zinkham法进行G6PD活性测定,其中G6PD酶活的正常值范围为6.8～20.5 IU/gHb;酶活低于6.8 IU/gHb的为G6PD缺乏型。采用南京建成生物公司的测试盒测定丙二醛(MDA)、H2O2、过氧化氢酶(CAT)和谷胱甘肽(GSH),采用HabermannHardt法测定溶血率。结果随着G6PD的酶活的缺失,MDA、GSH和H2O2在血液中含量均降低,相反,CAT的活性升高。G6PD缺乏型红细胞更容易受H2O2氧化损伤;脱氢表雄酮(DHEA)处理的红细胞中,DHEA抑制了G6PD酶活,并使红细胞对H2O2氧化性损伤敏感性增加。结论 G6PD酶活的缺失使红细胞中的抗氧化能力降低,是影响红细胞氧化应激敏感性的主要原因。
[Abstract]:Objective to investigate the relationship between glucose-6-phosphate dehydrogenase (G6PD) activity and oxidative injury in human erythrocytes and its effect on erythrocyte hemolysis. Methods A total of 267 blood samples from outpatients were selected and G6PD activity was determined by Zinkham method recommended by WHO. The normal range of G6PD enzyme activity was 6.8V 20.5 IU/gHb; activity lower than 6.8 IU/gHb. G6PD deficiency type was found. Malondialdehyde (MDA),) H _ 2O _ 2, catalase (CAT) and glutathione (GSH), were determined by HabermannHardt method. Results with the absence of enzyme activity of G6PD, the contents of MDA,GSH and H2O2 in blood decreased, on the contrary, the activity of CAT increased. G6PD deficient erythrocytes were more susceptible to oxidative damage of H2O2. In erythrocytes treated with dehydroepiandrosterone (DHEA), DHEA inhibited the activity of G6PD and increased the sensitivity of erythrocytes to H2O2 oxidative damage. Conclusion the lack of G6PD enzyme activity can decrease the antioxidant ability of erythrocytes, which is the main reason to affect the sensitivity of erythrocytes to oxidative stress.
【作者单位】：海南医学院生物化学与分子生物学教研室;海南医学院理学院;海南省安宁医院检验科;
【基金】：国家自然科学基金资助项目(编号:30960171) 海南医学院科研培育基金项目(编号:HY2010-002,HY2010-007)
【分类号】：R341

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