输尿管损伤后狭窄新西兰兔动物模型的构建
发布时间：2018-12-28 06:49
【摘要】：目的构建输尿管损伤动物模型,筛选输尿管损伤后纤维化进程中敏感的组织标志物,为进一步研究输尿管损伤后狭窄继发上尿路梗阻提供条件。方法采用双极电凝法构建输尿管损伤成年雄性新西兰兔动物模型。损伤后2周获取患侧输尿管组织及对侧正常输尿管组织,观察狭窄段的大体形态,并行苏木精-伊红(H-E)染色观察狭窄段纤维化程度。应用实时定量反转录-聚合酶链反应(RT-PCR)技术,检测内皮生长因子(EGF)、转化生长因子(TGF)-β、角化生长因子(KGF)、Ⅰ型胶原(ColⅠ)a1、ColⅠa2、Ⅲ型胶原(ColⅢ)和纤维连接蛋白(FN)在受累输尿管中的表达水平,并与对侧正常输尿管组织对比,寻找较敏感的纤维化指标。结果动物模型构建成功。动物模型构建过程中,狭窄侧输尿管组织中EGF、TGF-β、FN、ColⅠa1及ColⅠa2水平均显著高于正常侧输尿管组织(P值分别0.05、0.01)。结论双极电凝法构建输尿管损伤后狭窄新西兰兔动物模型可行。众多细胞因子中,EGF、TGF-β、ColⅠa1、ColⅠa2及FN在输尿管瘢痕形成中起重要作用,可能作为未来干预输尿管损伤后纤维化的药物靶点。
[Abstract]:Objective to establish an animal model of ureteral injury and screen sensitive tissue markers in the process of fibrosis after ureteral injury so as to provide conditions for further study of upper urinary tract obstruction secondary to stricture after ureteral injury. Methods the adult male New Zealand rabbit model of ureteral injury was established by bipolar electrocoagulation. The pathological and contralateral ureteral tissues were obtained 2 weeks after injury. The gross morphology of the stenosis was observed and the degree of fibrosis was observed by hematoxylin-eosin (H-E) staining. Real time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect endothelial growth factor (EGF), transforming growth factor (TGF) 尾 and keratinocyte growth factor (KGF), type 鈪，
本文编号：2393622
[Abstract]:Objective to establish an animal model of ureteral injury and screen sensitive tissue markers in the process of fibrosis after ureteral injury so as to provide conditions for further study of upper urinary tract obstruction secondary to stricture after ureteral injury. Methods the adult male New Zealand rabbit model of ureteral injury was established by bipolar electrocoagulation. The pathological and contralateral ureteral tissues were obtained 2 weeks after injury. The gross morphology of the stenosis was observed and the degree of fibrosis was observed by hematoxylin-eosin (H-E) staining. Real time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect endothelial growth factor (EGF), transforming growth factor (TGF) 尾 and keratinocyte growth factor (KGF), type 鈪，
本文编号：2393622
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